半边旗二萜化合物5F对HO-8910PM细胞中Nr1d1表达的影响  

Effect of 5F from Pteris semipinnata on expression of Nr1d1 in HO-8910PM cell line

作　　者：何太平[1] 吴科锋[2] 吕应年[2] 龚先玲[2] George Gong Chen 梁念慈[2] 

机构地区：[1]广东医学院检验学院,广东湛江524023 [2]广东天然药物研究与开发重点实验室,广东湛江524023 [3]香港中文大学威尔士亲王医院外科系

出　　处：《中国中药杂志》2009年第10期1268-1271,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(3987099);粤港科技合作项目(GHP/022/06)

摘　　要：目的:研究半边旗二萜化合物5F(5F from Pteris semipinnata L,PsL5F)对人高转移卵巢癌HO-8910PM细胞中核受体超家族成员1d1(nuclear receptor subfamily 1,group D,member 1,Nr1d1)表达的影响,探讨其抗肿瘤的可能作用机制。方法:培养HO-8910PM细胞,用0.1%DMSO,100μmol.L-1PsL5F分别处理HO-8910PM细胞24 h后,提取RNA和总蛋白,应用肿瘤基因芯片和荧光定量实时PCR检测PsL5F对Nr1d1 mRNA表达的影响;Western blot法分析PsL5F对Nr1d1蛋白表达水平的影响。结果:肿瘤基因芯片结果显示,100μmol.L-1PsL5F处理组中Nr1d1 mRNA表达水平是对照组的26.17倍,荧光定量实时PCR结果与芯片结果吻合,PsL5F处理组中Nr1d1 mRNA表达水平是对照组的(35.34±1.07)倍(P<0.01),并且PsL5F处理组中Nr1d1蛋白表达水平是对照组的(7.71±0.43)倍(P<0.01)。结论:PsL5F能明显上调HO-8910PM细胞中Nr1d1的表达,提示其与PsL5F抗肿瘤作用密切相关。Objective： To investigate the effects of PsL5F （ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid, an extract from Pteris semipinnata L） on the expression of nuclear receptor subfamily 1, group D, member 1 （Nr1d1） in highly metastatic ovarian carcinoma HO-8910PM cells, and its mechanisms. Method： Microarray Chip was used to examine the level of Nr1d1 mRNA expression on HO-8910PM ceils treated with PsL5F. Fluorescent quantitative real-time PCR assay and Western blot were performed to verify the effects of Psl.SF on Nr1d1 mRNA and protein expression. Result： After 24 h treatment of 100 μmol·L^-1 PsL5F, the mRNA and protein levels of Nrl dl in HO-8910PM cells were 35.34 ±1.07 and 7.71 ±0.43 times compared to those of control group（ P 〈 0.01, P 〈 0.01 ）, respectively. Conclusion： PsL5F can up-regulate siguificantly the expression of Nr1d1 in HO-891OPM cells. Antitumor effects and its mechanisms of PsL5F in HO-8910PM cells may be involved in the up-regulation of Nr1d1 expression.

关 键 词：半边旗 卵巢癌 Nr1d1 

分 类 号：R285.5[医药卫生—中药学]