加味三棱丸对子宫内膜异位症黏附分子ICAM-1，CD44表达的影响  

Inhibitory effect of Sanlengwan on expression of adhesion molecules ICAM-1,CD44 in rats of endometriosis

作　　者：李卓恒[1] 李傲[2] 陈刚[3] 何瑶[1] 王静[1] 徐晓玉[1,4] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]重庆工学院化学与生物工程学院,重庆400050 [3]重庆工商大学药物研究中心,重庆400067 [4]西南大学药学与中医药学院,重庆400716

出　　处：《中国中药杂志》2009年第10期1285-1289,共5页China Journal of Chinese Materia Medica

基　　金：重庆市科委应用基础研究项目[(2002)18号-99]

摘　　要：目的:研究加味三棱丸(SLW)对子宫内膜异位症移植物的抑制作用和探讨对黏附分子ICAM-1,CD44表达的影响。方法:采用子宫内膜自体移植法建立大鼠子宫内膜异位症模型,用游标卡尺、HE染色和免疫组化分别检测子宫内膜异位症移植物体积变化、异位内膜组织形态学改变和黏附分子ICAM-1,CD44的表达。结果:SLW 0.11,1.02 g.kg-1均可以明显减小子宫内膜异位症移植物的体积;显微形态显示:给药组与模型组比较其病理改变程度减轻,异位内膜为薄单层细胞排列而非腺体数目多,细胞呈多层排列,腺上皮呈扁平或矮立方形,未见假腺体改变;SLW 1.02,0.11 g.kg-1均能明显降低了异位子宫内膜黏附分子ICAM-1,CD44的蛋白表达。结论:SLW可显著抑制子宫内膜异位症移植物生长,其作用机制可能与抑制黏附分子ICAM-1,CD44的蛋白表达水平有关。Objective： To explore the effect of Sanlengwan （SLW） on adhesion molecules expression and the accretion in ectopic endometrium of rats. Method： Endometriosis was established by surgical implant of endometrial tissue which belong to its body. Forty EMS model rats were randomly divided into five groups： model control group, three dose of SLW groups and anastrozole group. Meanwhile, eight normol rats were used as the normal group. All the rats were treated for 4 weeks respectively, and the volume of grafts were measured by vernier caliper, morphological changes were measured by HE stain, and the adhesion molecules, ICAM-1 and CD44 protein, were also measured by immunohistochemical test before and after treatment of SLW. Result： SLW markedly reduced the volume of grafts, improve the morphological characters and decreased the expression of ICAM-1 and CD44 in ectopic endometrial tissue. Conclusion： SLW can inhibit the accretion of ectopic endometrium tissue of rats, and its mechanism might be associated with inhibiting the expression of ICAM-1 and CD44 protein.

关 键 词：加味三棱丸 子宫内膜异位症 黏附分子 ICAM-1 CD44 

分 类 号：R285.5[医药卫生—中药学]