MNNG对多杀菌素产生菌的诱变效应  

Mutagenic Effects of MNNG Treatment on the Spinosad Producing Strains

作　　者：宋炜[1,2] 熊犍[1] 郭伟群[2] 张晓琳[2] 宋渊[3] 

机构地区：[1]华南理工大学轻工与食品学院,广州510640 [2]国家粮食局科学研究院,北京100037 [3]中国农业大学生物学院,北京100193

出　　处：《中国生物防治》2009年第2期176-180,共5页Chinese Journal of Biological Control

基　　金：国家十一五科技支撑计划课题(2006BAD08802)

摘　　要：多杀菌素(spinosad)是刺糖多孢菌Saccharopolyspora sptnosa发酵产生的次级代谢产物,有显著杀虫活性。采用N-甲基-N'硝基-N-亚硝基胍(MNNG)作为诱变因子对刺糖多孢菌的孢子进行诱变处理,以高效液相色谱方法检测多杀菌素的发酵产量。研究结果表明:诱变剂量为2mg/ml,诱变40min时多杀菌素产量有较大幅度提高。通过诱变最终得到5株产量分别比出发菌株提高61.1%、80.3%、128.1%、69.9%和77.8%的突变株。Spinosad is a secondary metabolite with noticeable insecticidal activity maded from fermentation of Saccharopolyspora spinosa. The spores of Saccharopolyspora spinosa were mutated with N-methylN＇-nitro-N-nitrosoguanidin （MNNG）, and the yields of spinosad were detected by HPLC. Results showed that positive mutants with larger proportion were obtained when the spores of the parental strain were treated 40 min using 2 mg/ml MNNG. As a result, five high-producing strains were selected, and their spinosad titers were increased by 61.1%, 80.3%, 128.1%, 69.9% and 77.8% respectively.

关 键 词：刺糖多孢菌 多杀菌素 N-甲基-N'硝基-N-亚硝基胍(MNNG) 诱变 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]