石河子地区加工番茄坏死条斑病毒原的ELISA检测及品种抗病性鉴定  

Virus Detection of Necrotic Stripe Disease by ELISA and Resistance Identification of Processing Tomato Cultivars in Shihezi Area

作　　者：许文博[1] 黄家风[1] 阮涛[1] 

机构地区：[1]石河子大学绿洲农作物病害防控重点实验室,新疆石河子832003

出　　处：《中国蔬菜》2009年第10期41-45,共5页China Vegetables

基　　金：教育部"春晖计划"项目(Z2005-2-83004);石河子大学高层次人才科研启动基金(RCZX200516)

摘　　要：用番茄花叶病毒、蚕豆萎蔫病毒和黄瓜花叶病毒的单克隆抗体对田间表现坏死条斑症状的315个加工番茄自然病株进行了ELISA检测。结果表明,这3种病毒的检测侵染率很高,分别为69.3%、66.7%和75.1%,但其单一病毒的侵染率却很低,仅分别为1.5%、2.4%和8.5%。表明这3种病毒是石河子地区加工番茄上的主要病毒种类,其复合侵染极可能是造成田间大量病株呈现严重坏死条斑症状的主要因素。并且对田间自然发病的21个加工番茄品种的上述3种病毒带毒情况进行了ELISA检测,虽然供试品种体内带毒量都较高,但品种之间却表现出一定的抗病性差异。315 field samples of processing tomato showing necrotic stripe symptom were tested for virus by ELISA using monoclonal antibodies （MAbs） against Tomato mosaic virus （ToMV） , Broad bean wilt virus （BBWV） and Cucumber mosaic virus （CMV）, respectively. The result showed that the invading rate of ToMV, BBWV and CMV was 69.3 % , 66.7 % and 75. 1% , but sole infection rate of these 3 viruses was only 1.5 % , 2.4 % and 8.5 % , respectively. These indicated that the mixed infection was probably the main cause to induce necrotic stripe disease in Shihezi area. Situation of the above mentioned 3 viruses carried by 21 processing tomato cuhivars were tested by ELISA. The result showed there existed definite differences in resistance among cuhivars, although all tested cultivars had relatively high amount of viruses.

关 键 词：石河子地区 加工番茄 坏死条斑病 番茄花叶病毒 蚕豆萎蔫病毒 黄瓜花叶病毒 ELISA检测 抗病性鉴定 

分 类 号：S641.2[农业科学—蔬菜学]