应用UP-PCR进行瓜类保护地土壤镰孢菌多样性分析  

Diversity Analysis of Fusarium Species from Greenhouse Soil of Melon Crops with UP-PCR

作　　者：赵柏霞[1] 高增贵[1] 庄敬华[1] 张小飞[1] 赵辉[1] 郭培磊[1] 

机构地区：[1]沈阳农业大学植物保护学院,农业部北方农作物病害免疫重点开放实验室,辽宁沈阳110161

出　　处：《中国蔬菜》2009年第10期46-50,共5页China Vegetables

基　　金：辽宁省科技攻关计划项目(2006215004);辽宁省自然科学基金项目(20062018)

摘　　要：采用Universally Primed PCR(UP-PCR)技术对分离自辽宁省瓜类作物保护地土壤中的25株镰孢菌菌株和3株对照镰孢菌菌株进行了遗传多样性分析。筛选出6个扩增多态性好且稳定的通用引物,共扩增出73条带,其中多态性条带66条,占总条带数的90.4%。当相似系数为0.736时,可将28株镰孢菌菌株划分为8个类群,其中14株尖孢镰孢菌菌株聚为一个类群。Universally Primed PCR （UP - PCR） was conducted to analyze 25 strains of test Fusarium isolates and 3 strains of positive control Fusarium isolates. The results indicated that a total of 73 bands was amplified using 6 primers, of which 66 bands （90.4 % ） were polymorphic. The isolates were clustered into eight groups at the similarity of 0. 736 by cluster analysis, among which, 14 isolates of F. oxysporum were clustered into one group.

关 键 词：镰孢菌 UP-PCR 遗传多样性 

分 类 号：S436.42[农业科学—农业昆虫与害虫防治] S436.5[农业科学—植物保护]