Detection and evaluation of antibodies against neutrophil-activating protein of Helicobacter pylori in patients with gastric cancer  

作　　者：Min Long Jun Luo Yan Li Fang-Yin Zeng Ming Li 

机构地区：[1]Department of Medical Microbiology,School of Public Health and Tropical Disease, School of Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong Province, China [2]Department of Clinical Laboratory, Nanfang Hospital, Southern Medical University, Guangzhou 510515,Guangdong Province, China [3]School of Biotechnology, Southem Medical University, Guangzhou 510515, Guangdong Province, China

出　　处：《World Journal of Gastroenterology》2009年第19期2381-2388,共8页世界胃肠病学杂志（英文版）

基　　金：Supported by Grants from Guangdong Natural Science Foundation Project,5004750;National Key Development Project,973 Program 2002CB513206

摘　　要：AIM： To detect and evaluate the antibodies against Helicobacter pylori （H pylori） neutrophil-activating protein （HP-NAP） in patients with gastric cancer and other gastroduodenal diseases.METHODS： Recombinant HP-NAP was prepared from a prokaryotic expression system in Escherichia coll. Serum positivity and level of HP-NAP-specific antibodies in sera from 43 patients with gastric cancer, 28 with chronic gastritis, 28 with peptic ulcer, and 89 healthy controls were measured by rHP-NAP-based ELISA. rHP-NAP-stimulated production of interleukin-8 （IL-8） and growth-related oncogene （GROα） cytokines in the culture supernatant of SGC7901 gastric epithelial cells was also detected.RESULTS： The serum positivity and mean absorbancevalue of HP-NAP-specific antibodies in the gastriccancer group （97.7% and 1.01 ± 0.24） were significantly higher than those in the chronic gastritisgroup （85.7% and 0.89 ± 0.14, P 〈 0.005） and healthy control group （27.7% and 0.65 ± 0.18, P 〈 0.001）. The sensitivity and specificity of ELISA for the detection of HP-NAP-specific antibodies were 95.5% and 91.5%, respectively. HP-NAP could slightly upregulate IL-8 production in gastric epithelial cell lines but had no effect on GROα production.CONCLUSION： Infection with virulent H py/ori strains secreting HP-NAP is associated with severe gastroduodenal diseases, and HP-NAP may play a role in the development of gastric carcinoma, rHP-NAP- based ELISA can be used as a new method to detect H pylori infection. The direct effect of HP-NAP on gastric epithelial cells may be limited, but HP-NAP may contribute to inflammatory response or carcinogenesis by activating neutrophils.AIM:To detect and evaluate the antibodies against Helicobacter pylori(H pylori) neutrophil-activating protein(HP-NAP) in patients with gastric cancer and other gastroduodenal diseases.METHODS:Recombinant HP-NAP was prepared from a prokaryotic expression system in Escherichia coli.Serum positivity and level of HP-NAP-specific antibodies in sera from 43 patients with gastric cancer,28 with chronic gastritis,28 with peptic ulcer,and 89 healthy controls were measured by rHP-NAP-based ELISA.rHP-NAP-stimulated production of interleukin-8(IL-8) and growth-related oncogene(GROα) cytokines in the culture supernatant of SGC7901 gastric epithelial cells was also detected.RESULTS:The serum positivity and mean absorbance value of HP-NAP-specific antibodies in the gastric cancer group(97.7% and 1.01 ± 0.24) were signifi cantly higher than those in the chronic gastritis group(85.7% and 0.89 ± 0.14,P < 0.005) and healthy control group(27.7% and 0.65 ± 0.18,P < 0.001) .The sensitivity and specifi city of ELISA for the detection of HP-NAP-specific antibodies were 95.5% and 91.5%,respectively.HP-NAP could slightly upregulate IL-8 production in gastric epithelial cell lines but had no effect on GROα production.CONCLUSION:Infection with virulent H pylori strains secreting HP-NAP is associated with severe gastroduodenal diseases,and HP-NAP may play a role in the development of gastric carcinoma.rHP-NAPbased ELISA can be used as a new method to detect H pylori infection.The direct effect of HP-NAP on gastric epithelial cells may be limited,but HP-NAP may contribute to inflammatory response or carcinogenesis by activating neutrophils.

关 键 词：Helicobacter pylori Helicobacter pylorineutrophil-activating protein Gastric cancer PEPTICULCER Chronic gastritis 

分 类 号：R392-33[医药卫生—免疫学] R571[医药卫生—基础医学]