人胎胰腺的胰岛分离与胰岛细胞培养质量的实验研究  

Experimental Research on Islet Separationand Islet Cell Culture Quality in Human Embryo Pancreas

作　　者：袁柱[1,2,3,4] 吴光瑛[1,2,3,4] 何永蜀[1,2,3,4] 邵成民[1,2,3,4] 战雅[1,2,3,4] 

机构地区：[1]昆明医学院组胚教研室 [2]昆明医学院第一附属医院核医学科 [3]昆明医学院生物教研室 [4]昆明医学院第一附属医院放射科

出　　处：《昆明医学院学报》1998年第2期1-4,共4页Journal of Kunming Medical College

基　　金：云南省应用基础研究基金

摘　　要：取水囊引产的４例胎龄为１８～２８周的人胎胰腺，用胶原酶消化分离胰岛，分别对３，５，７，９，１１，１３ｄ培养的胰岛细胞进行形态学观察，并采用放射性核素１２５Ⅰ标记抗原作放射免疫测定（ＲＩＡ）其培养上清液中胰岛素及Ｃ－肽的含量．结果：经５～９ｄ培养的胰岛细胞，生长良好，细胞活率为９０％左右，其中大多数为β细胞．５，７，９ｄ培养液上清液中胰岛素的含量依次为６４７５，７２３及７２７０ＩＵ／Ｌ；Ｃ－肽的含量依次为６０５，６２及＞６ｍｇ／Ｌ．分别高于正常血清的两倍、一倍以上．经以同样方法培养的兔胰岛细胞用于四氧嘧啶实验性兔糖尿病模型治疗，兔糖尿病模型的血糖均值由（４７９７９±０９２３３）ｇ／Ｌ下降为（３３１９３±０４１１０）ｇ／Ｌ（Ｐ＜００１）；胰岛素由（２６５±１４）ＩＵ／Ｌ上升为（１３３８±１５）ＩＵ／Ｌ（Ｐ＜００５）．Taken from 4 cases of 18-to-28-week embryo induced by hydrostatic bag was pancreata, where islet was digested and separated with collagenase. Morphological observation was made on islet cells cultured respectively for 3d, 5d, 7d, 9d and 13d. Meanwhile the contents of insulin and C-peptide in the culture solution were measured by radioimmunoassay, with antigen labeled by radionuclide125I. Result: Islet cells cultured for 5-9 d grew well and cell survivals rate reached 90%, the majority of which were beta cells; insulin contents were respectively 64.75, 72.3 and 72.70 IU/L; and C-peptide contents were respectively 6.05, 6.2 and >6 mg/L, which were one or two times more than those in normal serum. Islet cells of rabbit embryo, which were cultured similarly with the above method, were applied in the experimental model treatment of rabbit diabetes, whose average blood sugar level decreased from (4.797 9±0.923 3) g/L to (3.319 3±0411) g/L (P<0.01) and whose insulin level increased from (2.65±1.4) IU/L to (13.88±1.5) IU/L (P<0.05). The results demonstrated that 5-9 d is appropriate for culturing islet cells for transplantation. 

关 键 词：细胞培养 胰岛细胞 移植 胚胎 糖尿病 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R587.1[医药卫生—基础医学]