Effect of Azoxystrobin on Oxygen Consumption and cyt b Gene Expression of Colletotrichum capsici from Chilli Fruits  

作　　者：CHEN Yu JIN Li-hua ZHOU Ming-guo 

机构地区：[1]College of Plant Protection, Nanjing Agricultural University~Key Laboratory of Monitoring and Management of Crop Diseases and PestInsects, Ministry of Agriculture, Nanjing 210095, P.R. China

出　　处：《Agricultural Sciences in China》2009年第5期628-631,共4页中国农业科学（英文版）

基　　金：sponsored by the National 973 Program of China (2006CB101907);the National 863 Program of China (2006AA10A211, 2008AA10Z414);the National Natural Science Foundation of China(30671048, 30671384)

摘　　要：Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b （cyt b）. Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid （SHAM, a known inhibitor of alternative respiration）, and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b （cyt b） gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.Azoxystrobin acts as an inhibitor of electron transport by binding to the Qo center of cytochrome b （cyt b）. Resistance to azoxystrobin was usually caused by the point mutation of cyt b gene or by the induction of alternative respiration. Oxygen consumption test for mycelia of Colletotrichum capsici showed that azoxystrobin inhibited mycelial respiration within 12 h; however, as time went on, the respiration of the mycelia recovered when the mycelia were treated with azoxystrobin and salicylhydroxamic acid （SHAM, a known inhibitor of alternative respiration）, and the oxygen consumption of the mycelia could not be inhibited. Meanwhile, cytochrome b （cyt b） gene expression increased with the recovery of mycelial respiration. The increased cyt b gene expression might play a role in the development of resistance to azoxystrobin in C. capsici.

关 键 词：QoI fungicides gene expression chili fungal respiration azoxystrobin resistance 

分 类 号：S828.2[农业科学—畜牧学] S436.413.1[农业科学—畜牧兽医]