Characterization of Side Cell Populations Obtained from Human Amnion Mesenchymal Cells  

作　　者：LI Ning PIAO Zheng-fu Mamoru Kobayashi Koji Sasaki DING Shu-qin Aiko Kikuchi Isao Kamo Norio Sakuragawa 

机构地区：[1]Institute of Hepatitis, Beijing You-an Hospital, Affiliated to Capital University of Medical Sciences, Beijing 100069, China [2]Department of Regenerative Medicine, Kitasato University,School of Allied Health Sciences, Nishi-hashimoto, Kanagawa 5-4-30, Japan

出　　处：《Chinese Journal of Biomedical Engineering(English Edition)》2009年第1期1-8,共8页中国生物医学工程学报（英文版）

基　　金：Scientific Research Common Program of Beijing Municipal Commission of Education;grant number:KM200810025009;Beijing Special Funds to Aid Returned Students;grant number:20080015

摘　　要：Human amnion mesenchymal cells （AMCs） contain muhipotent cells. To enrich such muhipotent stem cells, we applied to AMCs the new method for the isolation of side population （SP） cells used for the enrichment of muhipotent stem cells from many tissues. We succeeded in obtaining SP cells from AMCs （AMC-SP cells）. AMC-SP cells were found in 0.2 % of AMCs, irrespective of the length of pregnant period, ranging from 37 to 40 weeks. Cell cycle analyses suggested that AMC-SP cells belonged to a cell population that proliferated very slowly and/or was in a quiescent state in the amniotic membrane. Upon culturing, they proliferated with 40 to 80 cell doublings. However, they did not form colonies in a soft agarose culture, whereas HepG2 cells, representative human hepatoma cells formed many large colonies. These results suggest that AMC-SP cells that have considerable value for the use of regenerative medicine can be managed safely in vitro.Human amnion mesenchymal cells (AMCs) contain multipotent cells. To enrich such multipotent stem cells, we applied to AMCs the new method for the isolation of side population (SP) cells used for the enrichment of multipotent stem cells from many tissues. We succeeded in obtaining SP cells from AMCs (AMC-SP cells). AMC-SP cells were found in 0.2% of AMCs, irrespective of the length of pregnant period, ranging from 37 to 40 weeks. Cell cycle analyses suggested that AMC-SP cells belonged to a cell population that proliferated very slowly and/or was in a quiescent state in the amniotic membrane. Upon culturing, they proliferated with 40 to 80 cell doublings. However, they did not form colonies in a soft agarose culture, whereas HepG2 cells, representative human hepatoma cells formed many large colonies. These results suggest that AMC-SP cells that have considerable value for the use of regenerative medicine can be managed safely in vitro.

关 键 词：stem cells amnion mesenchyme side population cells mesenchymal cells cell therapy 

分 类 号：R685.2[医药卫生—骨科学] R644[医药卫生—外科学]