NTFs基因修饰的人胚OECs植入促进大鼠SCI神经再生及功能恢复的研究  

Recovery of rats spinal cord injury by transplantation of immortalized Ofactory enshething cells

作　　者：钟环[1] 蔡小娴[2] 陈继铭[1] 

机构地区：[1]广东医学院附属医院骨科 [2]广东医学院附属医院手术室,湛江524001

出　　处：《中国医疗前沿》2009年第6期13-14,共2页China Healthcare Innovation

摘　　要：目的探讨神经营养因子(NTF)基因修饰嗅鞘细胞(OECs)移植促进大鼠脊髓损伤后神经再生及功能恢复。方法SD大鼠制备成脊髓损伤动物模型。随机分为损伤对照组(A组)、OECs移植组(B组)和OECs神经营养因子基因修饰后移植组(C组)。治疗后2、4、6周,每组动物分别进行联合行为评分(GBS)、运动诱发电位(MEP)、感觉诱发电位(SEP)检查、双下肢功能测定(爬坡试验),评价脊髓损伤功能恢复情况。结果随时间的延长,B组GBS、MEP、SEP及下肢功能明显改善。与其它组相比较,差异有显著性,P<0.05。结论NTF基因修饰后移植在脊髓损伤区,能促进神经再生及功能恢复。Objective To study Recovery of rats spinal cord injury by transplantation on. immortalized Ofactory enshething cells（OECs）. Methods Spinal cord injured rats were divided into 3 goaeps. Group C consisted of the rat with treated with the transplantation of immortalized OECs modified byNTFs genes, group B of the rats with transplantation of OECs without immortalized., group A of the rats with without treatment At 2.4.6weeks the rats were examined with GBS score and neurological function were tested. Result The tintracord transplantation of immortalized OECs group were, improved the GBS score and neurological outcome of injured spinal cord. Conclusion The transplantation of immortalized OECs can improve the recovery of Spinal cord injured on rats.

关 键 词：嗅鞘细胞 基因修饰 脊髓损伤 移植 神经营养因子 

分 类 号：Q819[生物学—生物工程]