HPLC测定去纤软肝胶囊中芍药苷的含量  

Content determination of paeoniflorin in Quxian Ruangan Capsules by HPLC

作　　者：李润文[1] 

机构地区：[1]沈阳市第六人民医院,辽宁沈阳110006

出　　处：《中国医药导报》2009年第15期48-49,共2页China Medical Herald

摘　　要：目的:建立去纤软肝胶囊中芍药苷的含量测定方法。方法:采用HPLC法,色谱柱为Diamonsil C18(4.6mm×250mm,5μm),流动相为甲醇-0.2%磷酸溶液(40:60),流速为1.0ml/min,检测波长为230nm。结果:芍药苷在0.1168～1.1680μg范围内线性关系良好(r=0.9997),平均回收率为100.3%,RSD为0.92%。结论:该方法灵敏、准确、重复性好,为去纤软肝胶囊的质量控制提供了依据。Objective： To establish a method for the determination of paeoniflorin in Quxian Ruangan Capsules. Methods： HPLC was used with Diamonsil C18（4.6 min×250mm, 5μm） as chromatographic column and methanol-0.2% solution of phosphoric acid（40：60） as mobile phase, the flow rate was 1.0 ml/min and the detective wavelength was set at 230 nm. Results： There was a good linearity in the range of 0.116 8-1.168 0 μg（r=-0.999 7）, the average recovery was 100.3% with a RSD of 0.92%. Conclusion： This method is sensitive, accurate, reproducible, and provides a basis for the quality control of Quxian Ruangan Capsules.

关 键 词：HPLC 去纤软肝胶囊 芍药苷 含量测定 

分 类 号：R927.2[医药卫生—药学]