缺铁性贫血患儿红细胞密度及红细胞超氧化物歧化酶的相应分析  

Corresponding Analysis of Erythrocyte Density and Erythrocyte Superoxide Dismutase in Children with Ion Deficiency Anemia

作　　者：王健[1] 唐九义 

机构地区：[1]成都市第三人民医院 [2]都江堰市第二人民医院,610031

出　　处：《内蒙古中医药》2009年第3期35-37,共3页Inner Mongolia Journal of Traditional Chinese Medicine

摘　　要：目的：分析缺铁性贫血患儿红细胞密度及红细胞超氧化物歧化酶与疾病的关系。方法：采用密度梯度离心法将12例缺铁性贫血（IDA）和15例正常儿童的细胞超离心分离为各1/3上、中、下三层红细胞,并采用煌焦油蓝生理盐水法、邻苯三酚自氧化抑制法分别测定其红细胞的Ret分数数和E-SOD的活力。结果：对照组和病例组的Ret上中下三层分别为0.020,0.009,0.002和0.035,0.013,0.002（P〈0.01,P〈0.05和P〉0.05）;两组和E-SOD上中下分别为6349,5721,5301和6850,6165,5024（P〈0.05,P〈0.05和P〉0.05）。结论：提示上层为年轻红细胞,下层细胞逐渐衰老,IDA上层E-SOD受氧自由基影响,代偿活力增高,IDA红细胞包含了膜LPO增加和E-SOD代偿消耗的综合性病理过程。Objective： Analysis of erythrocyte density and erythrocyte superoxide dismutaes in children with ion deficiency anemia and illness get sick. Methods： In this study , red cells of 15 normal children and 12 IDA children were stratified into 3 layers respectively using density gradient centrifugation. Each of the layers consisted of 1/3 part of the sample. Rot fractions and E--SOD validity were measured by using normal saline brilliant cresyl method and O--trihydroxy benzene inhibition method respectively. Results： The top,middle and bottom layer Ret fractions of control group and IDA group are 0. 020,0. 009,0. 002 and 0. 035,0. 013,0. 002 respectively （P〈0.01 ,P〈0.05andP〉0.05） ; E--SOD of the three layers from top to bottom of the control group and IDA group are 6349,5721,5301 and 6850,6165,5024 respectively（P〈0.05 ,P〈0.05andP〉0.05）. Conclusions： Suggest that the top layers are young cells,the bottom layers are aging cells. Affected by oxygen free radicals,the E--SOD validity of top layer is compensatively high. IDA red cells experienced the pathological process of membrane LPO increasing and E--SOD consumption.

关 键 词：缺铁性贫血 红细胞密度 红细胞超氧化物歧化酶 

分 类 号：R446.11[医药卫生—诊断学]