THP-1巨噬细胞源性泡沫细胞模型的建立及鉴定  

Establishment and Identification for Macrophage-derived Foam Cell Model of THP-1 Cell Line

作　　者：王术玲[1] 潘华新[1] 王培训[1] 

机构地区：[1]广州中医药大学,广州510405

出　　处：《中药新药与临床药理》2009年第3期281-284,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的以人类单核细胞株THP-1建立泡沫细胞模型及建立鉴定模型的方法。方法以160nmol/L的佛波酯诱导THP-124h后分化成为巨噬细胞,继与80mg/L的氧化型低密度脂蛋白孵育48h,油红O染色后显微镜下观察细胞形态,采用HPLC-MS的方法测定细胞内胆固醇和胆固醇酯的含量。结果经油红O染色后,诱导建模的细胞内有大量的红色脂质颗粒存在;HPLC-MS测定结果显示,与正常单核细胞相比,建模的细胞内胆固醇和胆固醇酯含量均明显增加。结论THP-1细胞经佛波酯和氧化型低密度脂蛋白诱导后,能成功建立人类巨噬细胞源性泡沫细胞模型。Objective To establish a macrophage - derived foam cell model of human monocytic THP - 1 cell line and to identify the foam cell. Methods THP- 1 cells were differentiated into macrophages after induction with 160 nmol/L phorho]- 1 -myristate- 13- acetate for 24 hours and then incubation with 80 mg/L oxidized low density ]ipoprotein （oxLDL） for 48 hours. The differentiated cells were observed after oil red O staining under light microscope. High performance liquid chromatography/atmospheric pressure chemical ionization tandem mass spectrometry （HPLC- MS） was used for quantitative analysis of cellular cholesterol and eholesteryl esters contents. Results There was a large number of red lipid droplets in phorbol - 1 - myristate - 13 - acetate and oxLDL treated THP - 1 cells after oil red O staining under light microscope. Cellular contents of cholesterol and cholesteryl esters in the differentiated cells increased markedly compared with the normal THP- 1 monocytes by HPLC- MS assay. Conclusion A human monocyte- derived foam cell model has been established by incubating THP - 1 cells with 120nmol/L phorbol - 1 - myristate - 13 - acetate for 24 and then with 80 mg/L oxidized low density lipoprotein for 48 hours.

关 键 词：THP-1细胞 泡沫细胞模型 油红O染色 HPLC—MS法 

分 类 号：R285.5[医药卫生—中药学]