晚期糖基化终产物对人肝细胞株LO2增殖和凋亡的影响  

Effects of advanced glycation end products on proliferation and apoptosis of human heptical cell line LO2

作　　者：李海成[1] 何金花[1] 王明[1] 刘誉[1] 

机构地区：[1]暨南大学医学院生物化学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第2期143-147,156,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广州市科技计划项目(2002J1-C0361);暨南大学引进人才启动基金资助项目(51204004)

摘　　要：目的:研究晚期糖基化终产物(AGEs)对人正常肝细胞株LO2的作用。方法:以体外培养的LO2细胞株为研究对象,采用MTT法测定不同质量浓度(0、3.75、7.5、15、30、60 g/L)AGEs对LO2生长的抑制作用,应用流式细胞仪分析细胞的凋亡情况,RT-PCR分析不同质量浓度AGEs处理LO2后其IL-1β,TNF-α,HMGB1的基因转录水平变化。结果:当LO2细胞在AGEs质量浓度分别为3.75、7.5、15、30、60 g/L保温48 h后,细胞的增殖均受到显著性抑制,且抑制作用具有剂量依赖性。流式细胞仪分析发现AGEs促使LO2发生凋亡,凋亡率亦随AGEs浓度增高而增加。RT-PCR方法检测显示,AGEs使LO2细胞中IL-1β,TNF-α,HMGB1的基因mRNA的表达水平增高。结论:AGEs可抑制人正常肝细胞株LO2的增殖和诱导其凋亡,并能上调IL-1β,TNF-α和HMGB1基因的表达。Aim：To investigate the biological effects of advanced glycation end products （AGEs）on human normal heptical cell line LO2. Methods： Proliferative inhibition of LO2 by AGEs at different concentrations （0,3.75,7. 5,15,30,60 g/L） was accessed by using MTT assay. Cell apoptosis was analyzed by flow cytometry. The mRNA levels of IL-1β, TNF-α and HMGB1 were determined by RT-PCR. Results： AGEs at 3.75,7.5,15,30,60 g/L significantly inhibited proliferation of LO2 after incubation with AGEs for 48 h, and the inhibition showed dose-dependent fashions as well. AGEs were also found to be able to cause apoptosis in LO2 cells. Flow cytometry analysis showed that AGEs increased the proportion of apoptotic cells, while RT-PCR demonstrated that the mRNA levels of IL-1β, TNF-α and HMGB1 in LO2 cells were up-regulated by AGEs. Conclusion：AGEs inhibit proliferation, induce apoptosis and up-regulate the gene expression of IL-1β, TNF-α and HMGB1 in LO2 cells.

关 键 词：人肝细胞株 晚期糖基化终产物 小牛血清白蛋白 细胞凋亡 毒性 

分 类 号：R318.14[医药卫生—生物医学工程]