Morris水迷宫训练诱导大鼠海马轴突重塑  

Hippocampal axons remodeling was induced by Morris water maze in rats

作　　者：梁荫基[1] 宿宝贵[1] 潘三强[1] 韩辉[1] 湛湘丽[1] 吴盾[1] 

机构地区：[1]暨南大学医学院人体解剖学教研室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第2期157-161,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：广东省自然科学基金项目(05006067)

摘　　要：目的:观察Morris水迷宫训练后大鼠海马苔藓纤维分布的变化,探讨海马神经元轴突的可塑性。方法:2月龄雄性SD大鼠40只,随机分为水迷宫训练模型组20只,游水对照组20只。用Neo-Timm染色观察海马苔藓纤维的分布;免疫荧光组织化学方法检测海马微管相关蛋白tau-1的定位;Western blotting方法检测tau-1表达。结果:SD大鼠经6 d学习训练,其逃避潜伏期逐日缩短。与对照组大鼠相比,Neo-Timm染色显示模型组大鼠海马CA3区苔藓纤维延伸至锥体细胞层和起始层,tau-1免疫表达阳性产物除分布在透明层以外,锥体细胞层或起始层内有明显颗粒分布。Western blotting显示tau-1表达量增加。结论:结果表明Morris水迷宫训练可以诱导大鼠海马CA3区轴突的重塑,提示苔藓纤维系统可作为一个研究轴突重塑和认知关系的理想区域。Aim：To explore the plasticity of hippocampal neuronal axons, we investigated the remodeling of hippocampal mossy fiber by Morris water maze training in rats. Methods： Male SpragneDawley rats, 2 months were randomly divided into the model group （n = 20） and the control group （n = 20）. The distribution of mossy fiber was study by Neo-Timm stain. The distribution of microtube-associated protein tau-1 in the hippocampus CA3 region was observed by immunofluoreseenee stain between two groups. Expression of tau-1 was identified by Western blotting. Results： We demonstrated latency of platform becomes gradually shorter after six-day learning. Compared to the control group, mossy fiber terminals were significant increased in the CA3 stratum oriens region in model group. Distribution of tau-1 was obviously observed in stratum pyramidale and stratum oriens region. The expression of tau-1 in the model group was highly up-regulated compare to the control group. Conclusion：The results established learning and memory induced axonal remodeling of the hippocampal mossy fiber system in rat. Mossy fiber system could be an ideal region for researching the relationship between axonal remodeling and learning.

关 键 词：记忆 轴突可塑性 MORRIS水迷宫 tau-1 

分 类 号：R285.5[医药卫生—中药学]