出生前后慢性铝暴露对年轻大鼠海马神经颗粒素的影响  

Effects of chronic aluminum exposure from prenatal to postnatal stage on Ng in hippocampus of young rats

作　　者：南娟[1] 杜艳霞[1] 银杰[1] 赵永[5] 张占雀 刘鹏[1] 邢伟[2] 蔡葵[3] 蔡原[4] 时利德[1] 

机构地区：[1]中国医科大学基础医学院生理学教研室,沈阳110001 [2]中国医科大学基础医学院生物化学与分子生物学教研室,沈阳110001 [3]中国医科大学基础医学院神经生物学教研室,沈阳110001 [4]中国医科大学基础医学院毒理学教研室,沈阳110001 [5]哈尔滨医科大学人文社会科学学院法学教研室 [6]山西省芮城县人民医院

出　　处：《中华行为医学与脑科学杂志》2009年第3期226-228,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金资助项目（30371229）

摘　　要：目的研究出生前后不同浓度慢性铝暴露对年轻大鼠海马神经颗粒素（Ng）的影响，进一步探讨铝损害学习与记忆的突触机制。方法对照组、0．2％-Al组和0．4％-Al组大鼠各15只从孕期始分别自由饮用蒸馏水、Al^3＋浓度为15mmol·L^-1和30mmol·L^-1的A1Cl3水溶液制备出生前后慢性铝暴露模型；分别取学习和记忆的行为学测试后各组模型鼠9只，采用Western blot法检测海马细胞内Ng含量；组间资料统计用单因素方差分析，多重比较用LSD-t检验。结果对照组大鼠海马№目标条带的平均密度值为（96．99±6．52）；0．2％-Al暴露组为（83．59±7．17）；0．4％-Al暴露组为（62．44±14．26）。与对照组相比，0．2％-Al组海马Ng的表达明显降低（P=0．034），0．4％-Al组海马Ng表达显著性降低（P〈0．01），且铝暴露组间亦差异有显著性（P=0．002）。结论出生前后慢性铝暴露使№含量降低是其导致学习与记忆功能下降的突触机制之一。Objective To study the change of Ng in hippocampus of young rats after chronic aluminum exposure of different concentration from prenatal to postnatal stage, and to investigate the mechanism of aluminuminduced impairments to learning and memory. Methods Rats of control,low-dose and high-dose groups were treated from gestation with distiued water and AlCl3 water solution with concentration of 15 mmol·L^-1 and 30 mmol·L^-1 Al^3＋ respectively. The content of Ng was tested by Western-blot. Results The content of Ng in control group was 96.99 ±6.52 ,in 0.2% -Al group was 83.59 ± 7.17, in 0.4% -Al group was 62.44 ± 14.26. Compared with the control group,the content of Ng in 0.2%-Al group was remarkably decreased（P= 0. 034）and in 0.4%-Al group was significantly reduced（P 〈 0.01 ）, moreover,in the Al^3＋ exposed groups, the reduction was statistically significantly（P = 0.002）. Conclusion Aluminum exposure from prenatal to postnatal stage can reduce intracellular Ng content in hippocampus, which involve in impairing the ability of learning and memory.

关 键 词：铝暴露 神经颗粒素 出生前后 

分 类 号：R114[医药卫生—卫生毒理学]