双氢麦角碱对血管性痴呆小鼠海马乙酰胆碱代谢变化的影响  

Effect of dihydroergocriptine on the metabolic changes of acetylcholine in hippocampus of mice with vascular dementia

作　　者：靳玮[1] 冯志山[2] 吕佩源[1] 何春年[3] 翟金萍[3] 尹昱[4] 梁翠萍[1] 

机构地区：[1]河北省人民医院神经内科,石家庄050051 [2]河北省人民医院生化室,石家庄050051 [3]河北省人民医院病理科,石家庄050051 [4]河北省人民医院康复科,石家庄050051

出　　处：《中华行为医学与脑科学杂志》2009年第4期297-299,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家人事部留学人员科技活动择优资助项目（2001-119-1）

摘　　要：目的观察双氢麦角碱对血管性痴呆（VD）小鼠海马组织乙酰胆碱代谢变化的影响。方法采用反复脑缺血-再灌注法制备VD模型；通过跳台试验和水迷宫试验测试小鼠的学习记忆成绩；采用免疫组化和原位杂交技术观测海马胆碱乙酰转移酶（ChAT）及其mRNA表达的变化，并测定海马胆碱酯酶（AchE）的活性变化。同时，以双氢麦角碱作为干预药物，观测其对以上指标的影响。结果双氢麦角碱显著改善了VD小鼠的学习、记忆成绩[（24．13±4．59）S，（125．35±32．45）S；（184．08±44．74）s，（79．57±29．08）S；（68．32±9．26）S，（143．29±17．39）S；（57．05±5．02）S，（162．36±10．61）s；P〈0．01]。同时，模型组小鼠海马ChAT（0．0856±0．0089）及其mRNA（0．0780±0．0122）表达较假手术组[（0．1226±0．0149），（0．1286±0．0085）]显著下降（P〈0．01），其Ache的活性（14．53±0．86）也显著低于假手术组（17．15±1．02）（JP〈0．01）。药物组小鼠海马Ache活性（16．67±0．71）、CHAT（0．1152±0．0095）及其mRNA（0．1199±0．0090）表达有明显改善（P〈0．05）。结论海马胆碱能系统损伤参与了VD的形成，双氢麦角碱可能通过提高海马组织ChAT及其mRNA和AchE的活性，从而改善胆碱能系统的功能。Objective To study the effect of dihydroergocriptine on the metabolic changes of acetylcholine in hippocampus of mice with vascular dementia. Methods The mice model with VD were established by ischemi- a-reperfusion method;and the behavior abnormalities were investigated by step-down test and water-maze test. The changes of ChAT were studied by immunohistochemistry and in situ hybridization technique. The activities of AchE in hippocampus was detected. Furthermore, the effect of dihydroergocriptine on the above indexes was investigated. Results The grades of learning and memory of VD mice were obviously improved by dihydroergocriptine[ （24.13 ±4.59）s vs （125.35 ±32.45）s; （184.08 ±44.74）s vs （79.57 ±29.08）s; （68.32 ±9.26）s vs （143.29 ± 17.39） s; （57.05 ± 5.02） s vs （ 162.36 ± 10.61 ） s; P 〈 0.01 1. In model group, the expression of ChAT （0.0856 ±0.0089） and its mRNA （0.0780 ±0.0122） was lower than which （0. 1226 ±0.0149,0. 1286 ±0.0085） in shame group （ P〈0.01 ）,respectively. The activities of AchE （14.53 ± 0.86） decreased significantly （ P 〈 0.0l ）. In drug-treated group, the changes above mentioned were improved remarkably. Conclusions The abnormalities of cholinergic system in hippocampus might be involved into participating the formation of VD. Dihydroergocriptine could enhance the function of cholinergic system through promoting the expression of ChAT and its mRNA and increasing the activities of AchE.

关 键 词：血管性痴呆 双氢麦角碱 胆碱乙酰转移酶 

分 类 号：R285.5[医药卫生—中药学]