免疫荧光染色检测克隆牛X染色体组蛋白H3Lys4二甲基化修饰  

Immunofluorescence patterns of histone H3 lysine 4 dimethylation on X chromosomes of different cloned cattle

作　　者：曹更生[1,2] 张洪[1] 周文平[1] 李宁[2] 

机构地区：[1]河南大学生命科学学院,开封475004 [2]中国农业大学农业生物技术国家重点实验室,北京100193

出　　处：《遗传》2009年第6期611-614,共4页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30771216)资助

摘　　要：利用免疫荧光染色,检测胎儿成纤维细胞(FFB)和输卵管上皮细胞(FOV)来源的克隆牛X中期染色体组蛋白H3K4m2修饰状况。结果发现,这两种细胞系来源的克隆牛耳组织细胞系X染色体H3K4m2修饰与供体核FFB细胞系和常规繁殖牛耳组织细胞系的修饰基本一致,而与供体核FOV细胞系有较大差异。Using immunofluorescence staining, the patterns of histone H3 K4m2 of metaphase X chromosomes were measured in cloned cattle derived from FFB and FOV donor cells. The results demonstrated that the modification pattern of H3 K4m2 in the ear tissue of all clones was largely consistent with that of the donor cell line FFB and conventionally pro- duced cattle, but different from that of the donor cell line FOV.

关 键 词：免疫荧光染色 X染色体 H3 K4m2 表观遗传修饰 克隆牛 

分 类 号：Q343[生物学—遗传学]