虾夷扇贝遗传连锁图谱的初步构建  

The construction of a preliminary genetic linkage map in the Japanese scallop Mizuhopecten yessoensis

作　　者：刘卫东[1,2] 鲍相渤[2] 宋文涛[3] 周遵春[2] 赫崇波[2] 虞星炬[1] 

机构地区：[1]中国科学院大连化物所,大连116023 [2]辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,大连116023 [3]辽宁师范大学,生命科学学院,大连116023

出　　处：《遗传》2009年第6期629-637,共9页Hereditas（Beijing)

基　　金：国家海洋公益性行业科研专项(编号:200805037);辽宁省海洋与渔业科研计划项目(编号:200801);辽宁省科学技术计划项目(编号:2008203002)资助

摘　　要：用AFLP标记首次构建了虾夷扇贝遗传连锁图谱。用56对引物组合对父母本和52个F1代个体进行遗传连锁分析,共得到1855个标记,其中多态位点为598(32.2%)个,而354个符合孟德尔1:1分离比。用这些标记和23个偏分离标记(0.01<P<0.05)构建的虾夷扇贝遗传连锁图谱中,雄性连锁图谱包括178个标记,分布在22个连锁群上,总长度1263.0cM,标记平均间隔为8.1cM,图谱覆盖率为79.3%。雌性连锁图谱包括163个标记,22个连锁群,标记平均间隔为8.9cM,总长度1259.5cM,覆盖率为76.8%。标记在连锁群上分布不均匀并且出现大量0重组率标记。AFLP markers were used to construct the primary linkage map in a family of Mizuhopecten yessoensis. A total of 1 855 markers were generated in two parents and 52 progenies of the mapping family by using 56 AFLP primer combinations. Among the 1 855 markers, 598 were polymorphic and 354 were in agreement with the Mendelian segregating ratio of 1：1. Markers segregated according to Mendelian t：l ratio （P〉0.05） and 23 distorted markers （0.01 〈P〈0.05） were used in linkage analysis. The male map was composed of 178 markers in 22 linkage groups, covering a total of 1 263.0 cM with the average interval of 8.1 cM between markers, and the observed coverage was 79.4%. The female map was composed of 163 markers in 22 linkage groups, covering a total of 1 259.5 cM with the average interval of 8.9 cM between markers, and the observed coverage was 76.8%. AFLP markers showed an uneven distribution on the linkage group and there were a lot of markers with a distance of 0.

关 键 词：虾夷扇贝 遗传连锁图谱 AFLP标记 

分 类 号：S917.4[农业科学—水产科学] Q75[生物学—分子生物学]