甜蛋白Brazzein基因在番茄果实中的特异表达  

Fruit-specific expression of sweet protein Brazzein in transgenic tomato plants

作　　者：尹涛[1] 卢虹玉[2] 张上隆[1] 刘敬梅[3] 陈大明[1,4] 

机构地区：[1]浙江大学园艺系,杭州310029 [2]广东海洋大学食品科技学院,湛江524088 [3]北京市蔬菜研究中心,北京100089 [4]Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, USA

出　　处：《遗传》2009年第6期663-667,共5页Hereditas（Beijing)

基　　金：国家自然科学基金项目(No.30671430)资助

摘　　要：西瓜(Citrullus vulgaris S.)来源的AGPL1启动子在番茄(Lycopersicon esculentum L.)果实中具有较强的特异性驱动功能。将该启动子与甜味蛋白基因Brazzein融合构建植物表达载体,通过根癌农杆菌(Agrobacterium tumefaciens)介导法成功地进行了对番茄的遗传转化,获得转化植株。组织化学法、PCR特异扩增、Southern杂交分析及RT-PCR检测,表明Brazzein基因已整合到转基因番茄植株基因组中并且稳定表达。通过AGPL1果实特异启动子的调控,在不改变果实其他性状的前提下提高了番茄果实甜味品质,并为甜蛋白的生产提供经验。The AGPL1 （ADP-glucose pyrophosphorylase large subunit 1） promoter from watermelon （Citrullus vulgaris S.） has proved to exhibit fruit-specific expression patterns in tomato （Lycopersicon esculenturn L.）. A plant expression vector harboring sweet-taste protein, Brazzein, directed by AGPL1 promoter, was constructed and transferred into tomato plants through Agrobacterium-mediated transform methods. Histochemical staining assay, PCR screening, Southern blotting analysis and RT-PCR analysis showed that Brazzein gene was successfully integrated into the genome of transgenic tomato plants with stable expression. Sweet-taste fruits were produced under control of fruit-specific AGPL1 promoter, whereas other parameters of fruit quality were largely unchanged.

关 键 词：番茄 甜蛋白基因Brazzein 遗传转化 果实特异 AGPL1启动子 

分 类 号：Q943.2[生物学—植物学] S641.2[农业科学—蔬菜学]