丙型肝炎病毒总核心抗原测定方法及临床应用  

Clinical application of detection for total core antigen of hepatitis C virus

作　　者：谭德明[1] 聂东宋 彭小虎 杨锡琴 李凯 赵秀英[4] 欧阳奕[1] 曾立明[1] 周松辉 张贺秋[3] 周见远 

机构地区：[1]中南大学湘雅医院感染病科,湖南长沙410008 [2]湖南景达生物工程有限公司 [3]军事医学科学院基础医学研究所 [4]北京佑安医院 [5]湖南省岳阳市一医院

出　　处：《中国医师杂志》2009年第5期591-593,共3页Journal of Chinese Physician

基　　金：国家863重点项目（2006AA020907）

摘　　要：目的建立丙型肝炎病毒总核心抗原（总HCV-cAg）酶联免疫测定方法，并对相关的临床样品进行测定。方法通过对样品进行裂解处理，采用酶联免疫试剂盒（ELISA）检测总HCV-cAg，对201名抗-HCV阳性者血清进行总HCV—cAg检测，同时进一步作HCV-RNA检测，其中176例采用荧光定量PCR（FQ—PCR），25例采用逆转录PCR（RT—PCR）检测HCV．RNA。结果共检测201份血清标本，其中经PCR测定HCVRNA阳性88人份，阳性率43．8％；总HCV—cAg阳性71份，阳性率35．3％。经统计学分析，总HCV—cAg检测和HCVRNA检测的阳性率的差异无统计学意义。结论建立的总HCV—cAg酶联免疫测定方法适合临床应用，尤其适合在缺少RT-PCR或荧光定量PCR的中小医院使用。Objective To develop the technique to detect total core antigen of HCV（Total HCV-cAg） by Enzyme-Linked Immunosorbent Assay （ELISA） and apply it for clinical diagnosis. Methods 201 serum samples with anti-HCV antibody were detected total HCV-cAg after pre -treating the samples, then the sensitivity of results were compared with HCV RNA tests. Among them, 176 cases was determined by FQ-PCR, and 25 cases by RT-PCR for HCV-RNA. Results HCV RNA was found in sera from 88 of 201 samples （43.8 % ）. Total HCV-cAg was positive in 71 （35.3%） of 201 samples . There was no significant difference between the detection rate of HCV RNA by PCR and total HCV-cAg by ELISA. Conclusion Detection of total core antigen of HCV is suitable to be used as to diagnose HCV in clinic.

关 键 词：肝炎病毒/分析 肝炎抗原 丙型/分析 

分 类 号：R446[医药卫生—诊断学] R51[医药卫生—临床医学]