内皮细胞与系膜细胞相互作用对糖尿病肾病的影响  

The effect of the interaction of mesangial cells and endothelial cells on diabetic nephropathy

作　　者：周素娴[1] 赵晋晋[1] 雷闽湘[1] 

机构地区：[1]中南大学湘雅医院内分泌科,湖南长沙410008

出　　处：《中国医师杂志》2009年第5期597-599,共3页Journal of Chinese Physician

摘　　要：目的探讨高糖高脂环境下内皮细胞与系膜细胞相互作用对纤维连接蛋白（Fn）产生的影响及PAF在内皮细胞与系膜细胞相互作用的地位。方法内皮细胞与系膜细胞共培养和系膜细胞单独培养，分为对照组、甘露醇组、高糖高脂组、BN52021组，比较各组细胞上清液Fn、PAF含量。结果高糖高脂促进共培养和单培养Fn和PAF升高（P〈0．05）；高糖高脂条件下，共培养组Fn和PAF均较单培养组升高（P〈0．05），BN52021可抑制高糖高脂刺激的Fn升高（P〈0．05）。结论高糖高脂条件下，系膜细胞和内皮细胞存在异常的相互作用，这种作用促进Fn、PAF产生，高糖高脂刺激的Fn分泌增加部分与PAF有关。Objective To study the effect of high glucose and lysophosphatidylcholine （ LPC ） on the role of fibronectin and platelet activating factor （ PAF ） via the interaction of endothelial cells and mesangial cells. Methods The model of intercellular interaction between endothelial cells and mesangial cells was established and divided into 4 groups： control, mannitol, high glucose and LPC, and BN52021 group. The level of fibronectin and PAF were determined by enzyme linked immunosorbent assay in the culture media. Results The level of fibroneetin and＇PAF of high glucose and LPC group were higher than those of control group in co-culture and monolayer cell cul- ture （ P〈0.05 ）. Intervened by high glucose and LPC, the level of fibronectin and PAF of co-culture were higher than those from monolayer cell culture （ P〈0.05 ）. The level of fibrocentin in the BN52021 group was lower than that of high glucose and LPC （ P 〈0.05 ）. Conclusions Exposed to high glucose and LPC, endothelial cells and mesangial cells can interact with each other to produce more fibrocentin and PAF. The increase of fibronectin is partly concerned with PAF.

关 键 词：内皮细胞 肾小球膜 血小板活化因子 纤连蛋白类 糖尿病肾病 

分 类 号：R587.2[医药卫生—内分泌] R692.6[医药卫生—内科学]