苯系化合物对作业工人血浆热应激蛋白70抗体滴度及热应激蛋白70水平的影响  

The Measurement of Autoantibody to Heat Stress Protein and Heat Stress Protein in Plasma of Workers Exposed to Toluene and Xylene

作　　者：王秀玲[1] 金锡鹏[1] 周彤[1] 陈胜[2] 邬堂春[2] 

机构地区：[1]上海医科大学劳动卫生教研室 [2]同济医科大学职业医学研究所

出　　处：《劳动医学》1998年第2期69-72,共4页

摘　　要：目的：探讨苯系有机溶剂诱导人体产生ＨＳＰ７０及其抗体水平情况。方法：采用Ｗｅｓｔｅｒｎｂｌｏｔ和ＥＬＬＳＡ方法对职业性接触苯系化合物工人血浆中热应激蛋白ＨＳＰ７０和ＨＳＰ７０抗体水平进行了检测。结果：在接触甲苯和二甲苯的工人中血浆ＨＳＰ７０抗体阳性率明显高于对照组，且在甲苯组差别达到了统计学显著性水平。而ＨＳＰ７０蛋白含量在各组间比较没有差异。Total 138 workers in a paint-making factory were selected and divided into 3 groups,including 36 workers in toluene group,66 in xylene group and 36 in control group.Antibody to HSP70 in plasma was detected using responding purified HSPs with ELISA.The HSP70 was analyzed by Western Blot.It was showed that the positive occurrence rate of antibody to HSP70 in toluene group was higher than that in control group( P <0.05).No difference existed between xylene and control groups.The content of HSP70 in exposed groups was nearly equal to that in control group and no difference was observed among groups.The results suggested that antibodies to HSP70 may be used as a biomarker for exposure to toluene and xylene.

关 键 词：热应激蛋白 职业接触 甲苯 二甲苯 

分 类 号：R135.12[医药卫生—劳动卫生]