酒精影响新生大鼠脑细胞增殖的实验研究  

The experimental study of the influence of maternal alcohol consumption on cell proliferation in brain of new born rats.

作　　者：何旦莎[1] 吕明雄 

机构地区：[1]广州医学院神经科学研究所 [2]美国国家毒理研究中心

出　　处：《中国优生与遗传杂志》1998年第3期4-5,共2页Chinese Journal of Birth Health & Heredity

摘　　要：文应用流式细胞术检测新生１天大鼠的大脑、小脑和脑干脑细胞增殖特性。观察了大鼠在妊娠期间喂以含酒精食物对其新生仔鼠脑细胞增殖的影响。从孕１天至分娩，给孕大鼠喂养含酒精的流质饮食，对照组、低浓度酒精组和高浓度酒精组其食物中酒精源性热卡占总热卡的比例分别为０％、１８％和３６％。结果显示，高浓度酒精组大脑和脑干细胞增殖明显低于对照组和低浓度酒精组，提示酒精干扰新生仔鼠脑细胞增殖，这可能是导致“胎儿酒精中毒综合征”患儿智力和行为受损的病理机制之一。Effect of prenatal alcohol exposure on brain cell proliferation in new born rats was investigated by means of flow cytometry. The pregnant rats consumed an ethanol containing liquid diet containing 0% (control group), 18% (low dose ethanol) and 36% (high dose ethanol) ethanol derived calories from first day of gestation until parturition. The level of cellular proliferation of cerebrum, cerebellum and brain stem was analyzed in the 1 day old rats. Cell proliferation activity of cerebrum and brain stem in high dose group was significantly decreased as compared to the control or low dose group. Our observations suggested that alcohol related derangement in the patterns of proliferation of the brain cells probably contributed the pathogenesis of the intellectual and behavioral impairment in FAS children.

关 键 词：酒精 细胞增殖 流式细胞术 脑 新生大鼠 

分 类 号：R994.4[医药卫生—毒理学] R992[医药卫生—药学]