白细胞介素17抗体对哮喘小鼠中性粒细胞的影响  

Effects of IL-17 neutralization antibody on polymorph-nuclear of asthma in mice

作　　者：李洧[1] 叶茜[1] 高顺翠[1] 李道卫[1] 姜淑娟[1] 朱玲[1] 

机构地区：[1]山东大学附属省立医院呼吸内科,济南250021

出　　处：《山东大学学报（医学版）》2009年第3期43-47,共5页Journal of Shandong University:Health Sciences

基　　金：山东省医药卫生科学研究资助项目(2005年第21号)

摘　　要：目的观察白细胞介素17(IL-17)抗体对哮喘小鼠中性粒细胞(PMN)的影响。方法随机将40只雄性昆明小鼠分为对照组(A组)、哮喘组(B组)、IL-17抗体组(C组)、地塞米松组(D组),每组10只,采用卵蛋白致敏和激发方法建立哮喘模型并给予相应治疗,对血PMN分离纯化检测凋亡率;采用支气管肺泡灌洗液(BALF)进行细胞分类计数,肺组织病理切片HE染色观察炎性细胞浸润;采用免疫组化法检测中性粒细胞髓过氧化物酶(MPO)表达水平。结果C组外周血PMN凋亡率明显高于B组,BALF中Neu计数及肺组织免疫组化MPO阳性细胞数明显低于B组;D组外周血PMN凋亡率及BALF中Neu计数与B组差异无统计学意义,肺组织免疫组化MPO阳性细胞数明显高于B组。结论IL-17抗体有利于缓解哮喘小鼠PMN引起的炎症反应。Objective To investigate the effects of IL-17 neutralization antibody on polymorph-nuclear （PMN） of asthma mice. Methods A total of 40 Kunming mice were randomly divided into four groups： the control group （group A）, the asthma group （group B）, the IL-17 neutralization antibody group （group C）, and the Dex group （group D）. Mice were sensitized and challenged with ovalbumin（OVA） to establish asthma models, and groups C and D were injected with the IL-17 neutralization antibody and Dex respectively. Peripheral blood PMN was isolated and purified to observe the apoptesis percentage. The total cell number and differentiation cell number were counted in the bronehoalveolar lavage fluid （BALF） . The pathological characters of lung tissues were observed using HE staining. Myeloperoxidase （MPO） expression was detected by technique. Results Percentage of the PMN apoptosis in group C was significantly higher than that in group B, while the number of Neu in BALF and MPO level in lung tissues were significantly decreased. Compared with group B, the percentage of PMN apoptosis and number of Neu in BALF in group C had no significant differences, while levels of MPO were significantly increased. Conclusion IL-17 neutralization antibody may inhibit asthmatic airway inflammation caused by PMN.

关 键 词：哮喘 白细胞介素17 中性白细胞 小鼠 

分 类 号：R562.2[医药卫生—呼吸系统]