辛伐他汀对人脐静脉内皮细胞分泌的细胞因子TNF-α、IL-6及uPA/uPAR的抑制作用  

Inhibitory effect of simvastatin on expression of Cytokine TNF-α,IL-6 and uPA/uPAR secreted by HUVECs

作　　者：窦姗姗[1] 邓景惕[1] 季冉[1] 扈燕来[1] 张伟红[2] 张立平[1] 

机构地区：[1]山东大学医学院人体解剖学教研室,济南250012 [2]山东大学第二医院手术室,济南250033

出　　处：《山东大学学报（医学版）》2009年第4期1-4,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y99C03)

摘　　要：目的应用脂多糖(LPS)刺激人脐静脉内皮细胞(HUVECs)后,检测细胞因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和尿激酶型纤溶酶原激活物及其受体(uPA/uPAR)的mRNA和蛋白含量的变化及辛伐他汀对其表达的影响。方法实验分为12h组和24h组,各组又分为空白对照组、脂多糖(100ng/mL)组、辛伐他汀(5μg/mL)组、辛伐他汀(5μg/mL)和脂多糖(100ng/mL)共作用组,用实时荧光定量PCR和ELISA方法测定12h组、24h组TNF-α、IL-6及uPA/uPAR的mRNA和蛋白表达的变化。结果与对照组相比,脂多糖组TNF-α、IL-6及uPA/uPAR的mRNA表达和上清液中IL-6蛋白的表达均明显增加(P均<0.05);与脂多糖组相比,辛伐他汀和脂多糖共作用组TNF-α、IL-6及uPA/uPAR的mRNA表达和上清液中IL-6蛋白的表达均明显降低(P均<0.05)。结论辛伐他汀可抑制HUVECs分泌的细胞因子TNF-α、IL-6及uPA/uPAR的表达。Objective To explore the effect of simvastatin on expressions of TNF-α, IL-6, uPA/uPAR mRNA and protein in HU- VECs treatment with LPS. Methods Cultivated HUVECs were divided into two groups： the 12 h group and the 24 h group. Then they were sub-divided into four groups： the control group, the LPS（100 ng/mL）group, the simvastatin（5μg/mL） group, and the LPS and simvastatin group. Expressions of TNF-α, IL-6, uPA/uPAR mRNA and protein were determined using real-time quantitative PCR and ELISA. Results Compared with the control groups, expressions of TNF-α, IL-6, uPA/uPAR mRNA and IL-6 in the supernatant were signitlcanfly increased in the LPS groups （ P 〈 0.05）. Compared with the LPS groups, expressions of TNF-ct, IL-6, uPA/uPAR mRNA and IL-6 in the supematant were statistically decreased in the LPS and simvastatin groups （P 〈 0.05）. Conclusion Sirnvastatin can inhibit expressions of TNF-α, IL-6 and uPA/uPAR secreted by HUVECs.

关 键 词：人脐静脉内皮细胞 脂多糖 辛伐他汀 细胞因子 

分 类 号：R543.5[医药卫生—心血管疾病]