大鼠肺移植受体OB与细胞凋亡关系的初步研究  

Correlation between cell apoptosis and obliterate bronchiolitis in rats following allogeneic lung transplantation

作　　者：魏煜程 王滋宗[1] 杨荣华[1] 沈毅[1] 

机构地区：[1]青岛大学医学院附属医院胸外科,山东青岛266003

出　　处：《山东大学学报（医学版）》2009年第4期21-25,共5页Journal of Shandong University:Health Sciences

摘　　要：目的探讨大鼠肺移植受体闭塞性细支气管炎(OB)与细胞凋亡的关系。方法采用大鼠气管异位移植模型模拟肺移植术后OB。随机分为对照组、移植组和免疫抑制剂治疗组,分别于术后7、14和28d取出移植物。原位末端标记法(TUNEL)检测移植气管细胞凋亡情况;免疫组织化学EnVison法检测移植气管Caspase-3和Bax基因蛋白的表达。结果①动物模型气管HE染色检测到术后排斥反应,与OB组织学改变符合;②TUNEL检测到大鼠OB模型存在细胞凋亡,免疫抑制剂治疗组较移植组细胞凋亡数少,移植中后期两组差异有统计学意义(P<0.05);③免疫组织化学检测到大鼠OB模型Caspase-3、Bax基因蛋白表达在移植组和免疫抑制剂治疗组增高,移植中后期两组差异有统计学意义(P<0.05)。结论①大鼠肺移植受体OB与细胞凋亡相关;②抑制细胞凋亡可治疗肺移植受体OB。Objective To explore the correlation between apoptosis and obliterate bronchiolitis （OB） after allograft lung trans- plantation in rats. Methods Heterotopic trachea transplantation was performed on Wistar rats with OB after allograft lung trans- plantation. Animal models were randomly divided into the control group, the transplantation group and the immunosuppressant treatment group. All allograftic trachea were taken out on the 7th, 14th and 28th d after transplantation. TUNEL was used to de- tect apoptosis, and an EnVison inanunohistochemical method was used to detect expressions of Caspase-3 and Bax in the grafts. Results ① Animal models showed the same histological changes as OB after allograft lung transplantation. ②TUNEL test showed that apoptosis in the OB model and the immunosuppressant treatment group had less apoptosis than that in the transplanta- tion group（ P 〈 0.05）. ③ HIS showed that expressions of Caspase-3 and Bax were increased in OB models and were less in the immunosuppressant treatment group than in the transplantation group, especially in the later period（ P 〈 0.05）. Conclusion OB after allografi lung transplantation is related to apoptosis. Suppressing apoptosis maybe a treatment method for OB after al-lograft lung transplantation.

关 键 词：肺移植 闭塞性细支气管炎 细胞凋亡 大鼠 Wistar 

分 类 号：R655.3[医药卫生—外科学]