胰岛素样生长因子-1对背根神经节神经元谷氨酸损伤的保护作用  

Neuro-protective effects of insulin-like growth factor 1 on cultured dorsal root ganglion neurons with neurotoxicity induced by glutamate

作　　者：张凤军[1] 王丽红[1] 刘真[1] 邢子英[1] 王怀经[1] 李振中[1] 

机构地区：[1]山东大学医学院解剖学教研室,济南250012

出　　处：《山东大学学报（医学版）》2009年第4期38-41,45,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金重点项目(Z2006D05;Z2006C06)

摘　　要：目的探测胰岛素样生长因子-1(IGF-1)对背根神经节(DRG)神经元谷氨酸(Glu)损伤的保护作用。方法Wistar胎鼠DRG神经元分散培养48h后,用Glu(200μmol/L)造成神经元损伤,并同时给予IGF-1(10nmol/L),观察添加IGF-1和未添加IGF-1孵育的Glu损伤的神经元的活细胞生长状况,并用流式细胞仪检测神经元的凋亡率,用共聚焦激光扫描显微镜(CLSM)检测细胞内钙离子荧光强度。结果用IGF-1孵育的神经元生长状况良好,凋亡率、细胞内钙离子荧光强度均低于无IGF-1孵育的标本。结论IGF-1可通过减低钙离子内流,抑制细胞凋亡,从而对Glu损伤的DRG神经元产生保护作用。Objective To determine the neuro-protective effects of insulin-like growth factor 1 （IGF-1） on cultured dorsal root ganglion （DRG） neurons injured by glutamate （Glu）. Methods DRG neurons of Wistar rat embryos were cultured for 48 h in vitro and then exposed to Glu （200 μmol/L） with or without IGF-1 （ 10 nmol/L）. The living cells were observed with an inverted contrast microscope. The cultures were processed for detecting the apoptosis rate using flow cytometry and for detecting the fluo- rescent intensity of intracellular Ca^2＋ using confocal laser scanning microscopy （CLSM）. Results Survival status of the DRG cells with IGF-1 incubation was better than that without IGF-1 incubated cultures. The apoptosis rate and fluorescent intensity of Ca^2＋ were lower in cultures with IGF-1 than those without. Conclusion IGF-1 may reduce intracellular Ca^2＋ concentration, in- hibits neuronal apeptosis, and protects the DRG neurons from neurotoxicity induced by Glu.

关 键 词：胰岛素样生长因子-1 神经节 神经元 谷氨酸 大鼠 WISTAR 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] R-332[医药卫生—基础医学]