RP-HPLC法分析不同种属和采集地点苔藓植物中双联苄类化合物成分(英文)  

Determination of Bisbibenzyls in Bryophytes Collected from Different Locations by RP-HPLC

作　　者：吕蓓蓓[1] 邢杰[1] 娄红祥[1] 

机构地区：[1]山东大学药学院,济南250012

出　　处：《中国天然药物》2009年第3期217-222,共6页

基　　金：supported by the National Basic Research Program of China (No. 2006CB708511);the National Natural Science Foundation of China (No. 30730109)~~

摘　　要：目的:建立一种反相高效液相色谱法以快速分析四种苔藓植物中双联苄类化合物, 并对不同种属不同采集地点的苔藓植物中双联苄类化合物进行分析比较, 以考察种属及采集地点对该类化合物的种类和含量影响。方法:采用 Hypersil C18柱(150 mm×4.6 mm,I.D. 5μm)分离, 以乙腈-水(各含0.05%甲酸)为流动相, 线性梯度洗脱,于280 nm波长下检测。结果:共检测出6种双联苄类化合物, 并且种属和采集地点差异对该类化合物的种类和含量有不同程度的影响。结论:建立了一种有效的分析及测定不同苔类植物中双联苄类化合物的方法,并可为苔藓植物化学成分研究提供参考信息。AIM： To develop a rapid high-performance liquid chromatographic method for the determination of bisbibenzyls in four bryophytes. The effects of species and sample locations on the bisbibenzyl composition and con- tent were also evaluated. METHOD： Chromatographic separation was achieved on a Hypersil C18 column （150 mm~ 4.6 mm, I.D. 5 Ixm） with acetonitrile-water gradient elution （each containing 0.05% formic acid）. UV detection was per- formed at 280 nm. RESULTS： A total of 6 bisbibenzyls were detected in four bryophytes, and the results show that the sample locations and species have an influence on the bisbibenzyl composition and the content of each bisbibenzyl. CONCLUSION： This method not only provides a useful tool for analyzing and quantifying the bisbibenzyls but also provides useful information on the phytochemical research of these bryophytes.

关 键 词：高效液相色谱法 苔藓植物 双联苄类化合物 

分 类 号：TQ460.72[医药卫生—药物分析学]