子宫内膜癌错配修复基因hMLH1的表达和启动子甲基化研究  

Mismatch Repair Gene hMLH1 Expression and Promoter Methylation in Endometrial Carcinoma

作　　者：舒珊荣[1] 柯佩琪[1] 李小毛[2] 李美香[3] 王莉[3] 费慧[1] 

机构地区：[1]中山大学 附属第一医院妇产科 ,广东广州510630 [2]中山大学 附属第三医院妇产科 ,广东广州510630 [3]中山大学肿瘤防治中心,广东广州510630

出　　处：《中山大学学报（医学科学版）》2009年第3期241-245,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技攻关项目(2005B34201022)

摘　　要：【目的】探讨子宫内膜癌组织中错配修复基因hMLH1的表达和启动子甲基化状态与子宫内膜癌发生的关系。【方法】应用免疫组化链霉素抗生物素-过氧化物酶法(S-P法)和甲基化特异性聚合酶连反应(MSP)方法,检测37例子宫内膜癌组织和20例正常增生期子宫内膜组织中hMLH1蛋白的表达和启动子甲基化状态。【结果】37例子宫内膜癌组织和20例正常增生期子宫内膜组织hMLH1基因蛋白表达分别为51.4%(19/37),85%(17/20);基因启动子甲基化分别为43.2%(16/37),15%(3/20)。发生甲基化的16例子宫内膜癌组织14例蛋白表达阴性,发生甲基化的3例正常增生期子宫内膜组织2例蛋白表达阴性。hMLH1蛋白表达与组织学分级(G1,G2,G3)有关,与肿瘤肌层浸润、淋巴结转移、国际妇产科协会(FIGO)分期和宫颈浸润无相关性,hMLH1的甲基化与上述临床病理因素均无相关性。【结论】hMLH1基因启动子甲基化与其蛋白表达缺失密切相关,hMLH1基因在子宫内膜癌的发生过程中发挥重要作用。[Objective] To explore the correlation between the mismatch repair gene hMLH1 protein expression and promoter methylation with endometrial carcinoma. [Methods] The protein expression of hMLHI and the status of promoter methylation were detected by immunohistochemistry and methylation specific PCR （MSP） in the tissues of endometrial carcinoma and normal proliferative endometrium. [Results] The protein expression rate of hMLH1 was 51.4% （19/37） in endometrial carcinoma and 85%（17/20） in normal proliferative endometrium, respectively. The DNA methylation rate was 43.2%（16/37） and 15%（3/20）in endometrial carcinoma and normal proliferative endometrium, respectively. The hMLH1 was not expressed in 14 of 16 methylated cases of endometrial carcinoma and 2 of 3 methylated eases of normal proliferative endometrium. The protein expression of hMLH1 was correlated with histological grade （G1, G2, G3） of endometrial carcinoma. No correlation was found between muscular layer invasion, lymph metastasis, FIGO stage and cervical invasion with bMLH1 protein expression, hMLH1 methylation did not correlate with such clinicopathological characteristics. [Conclusions] hMLH1 methylation was associated with the absence of hMLH1 protein expression. The hMLH1 involved in carcinogenesis of endometrial carcinoma.

关 键 词：子宫内膜癌 HMLH1基因 表达 启动子甲基化 

分 类 号：R71[医药卫生—妇产科学]