大黄蛰虫丸对大鼠肝星状细胞活化增殖的影响  

Influence of Dahuang Zhechong Pill on Proliferation of Rat Hepatic Stellate Cells

作　　者：潘志恒[1] 成家茂[2] 李永伟[1] 何宏文[3] 谢瑶[3] 何义华[1] 

机构地区：[1]中山大学附属第三医院中医科,广东广州510630 [2]大理医学院人体解剖学教研室,云南大理671000 [3]中山大学人体解剖学教研室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2009年第3期250-254,274,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家中医药管理局基金项目(04-05JP51);广东省中医药管理局科研基金滚动项目(2007104)

摘　　要：【目的】观察大黄蛰虫丸对经旁分泌和自分泌途径激活大鼠肝星状细胞活化增殖情况的影响。【方法】采用Nycodenz分离液非连续密度梯度离心法同步分离急性CCl4损伤模型大鼠和正常大鼠肝的枯否细胞(KC)和星状细胞(HSC)。在制备正常鼠血清和DHZCW药物血清的基础上,将它们分别加入KC和HSC培养板内作用后制备成正常鼠KC条件培养液(NKCCM)、损伤鼠KC条件培养液(KCCM)以及正常鼠HSC条件培养液(HSCCM)。将上述条件培养液分别干预HSC24h、48h和72h,然后采用MTT法和3H-TdR掺入法分别检测比较各组HSC细胞活化增殖情况。【结果】损伤鼠不含药组和正常KC对照组比较,HSC活化增殖情况显著性增高(P<0.01),损伤鼠含药组HSC活化增殖则显著降低(P<0.01,P<0.05),且其抑制效应随着含药血清浓度的升高逐渐增强;而正常鼠HSC不含药组和正常鼠HSC含药组各组间比较均无显著性差异(均P>0.05)。【结论】DHZCW对经旁分泌途径激活大鼠HSC活化增殖有明显的抑制作用,且作用效应与血清药物浓度间存在剂量依赖关系,但对经自分泌途径激活大鼠HSC活化增殖未见明显抑制作用。[Objective] To observe the effects of Dahuang Zhechong Pill （DHZCW） on the proliferation of rat hepatic stellate cells （HSCs） activated by paracrine and autocrine pathway. [Methods] The CCl4-injured and normal rat Kupffer cells （KC）, hepatic stellate cells （HSC） were separated by non-continuous density gradient centrifugation of Nycodenz medium. After the common rat and DHZCW drug sera were prepared, they were added respectively into KC and HSC culture plates for normal KC conditioned medium （NKCCM）, injured rat KC conditioned medium （KCCM） and HSC conditioned medium （HSCCM）. After HSCs were interfered for 24, 48 and 72 h by those conditioned media, the changes of proliferative HSCs in all groups were observed and compared by the methods of methyl thiazolyl tetrazolium （MTT） and 3H-thymidine （3H-TdR） incorporation assay. [Results] Compared to HSCCM group, the multiplication of HSCs increased markedly in KCCM without drug group （P 〈 0.01）. The activation and proliferation of HSC decreased obviously in KCCM with drug group （P 〈 0.05） and the inhibitory effect enhanced as the concentration of sera with drug increased. There were no significant differences between HSCCM without drug group and HSCCM with drug group （P 〉 0.05）. [Conclusions] DHZCW can obviously inhibit the proliferation of rat HSCs activated by paracrine pathway, and there is a concentration-dependent relationship between the inhibition effect and the drug dose. However, there was no remarkably inhibition effect on the proliferation of rat HSCs activated by autocrine pathway.

关 键 词：大黄蛰虫丸 旁分泌 自分泌 肝星状细胞 增殖 

分 类 号：R286[医药卫生—中药学]