HSV-2333株在Vero细胞中持续感染及增殖特性研究  

Study on Infection and Proliferation of HSV-2 in Veto Cells

作　　者：孙朝晖[1] 石玉玲[1] 冼江[2] 王捷[2] 杨慧兰[3] 

机构地区：[1]广州军区广州总医院实验科,广东广州510010 [2]广州军区广州总医院检验科,广东广州510010 [3]广州军区广州总医院皮肤科,广东广州510010

出　　处：《医学临床研究》2009年第5期753-756,共4页Journal of Clinical Research

基　　金：广东省自然科学基金（7000065）;中国博士后科学基金（20070410831）;广东省医学科研基金（A2007477）

摘　　要：[目的]探讨单纯疱疹病毒Ⅱ型（HSV-2）在非洲绿猴肾细胞（Vero细胞）中感染及增殖的特性。【方法】将HSV-2接种于Vero细胞中培养和传代，制备电镜样品观察，于不同时间收获病毒液并测毒力，进行pH值、血清以及在Vero细胞传代、不同感染复数等不同培养条件的摸索。【结果】pH值、残留牛血清是HSV-2在Vero细胞培养的影响因素，最佳收毒时间为36-72h。在Vero细胞上培养病毒3-5代，病毒毒力较高。【结论】建立了HSV-2在Vero细胞上培养增殖的初步方法，为下一步建立HSV-2的潜伏与激发细胞模型打下基础。[Objective] To study the condition of HSV-2 infection and proliferation with high yield in Vero cells. [Methods] HSV-2 was inoculated into Vero cells to culture and subculture. Harvest virus suspension in different time and the titration of virus was tested. The condition of HSV-2 propagation was investigated. [Results] The results showed that the propagation of HSV-2 in Vero cell was influenced by pH value and quantity of calf serum. The best time of virus harvest was 36-72 hours after inoculation. Virus titration of 3 -5 passages of HSV-2 in Vero cell was the highest in 10 passages. [Conclusion] The procedure for HSV-2 proliferation with high virus yields has been established and provides the basis for the study on latency and reactivation of HSV-2 in vitro.

关 键 词：VERO细胞 单纯病毒属 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]