不同产地半边旗中11β-羟基-15-氧-对映贝壳杉-16-烯-19-酸19-β-D-葡萄糖的含量测定  

Quantitative Analysis of 11β-hydroxy-15-oxo-ent-kaur-16-en-19-oic Acid 19-β-D-Glucoside from Pteris Semipinnata L. in Diferent Areas by HPLC

作　　者：龚先玲[1] 陈志红[2] 梁念慈[1] 

机构地区：[1]广东医学院广东天然药物研究与开发重点实验室,广东湛江524023 [2]广东医学院分析中心,广东湛江524023

出　　处：《河南中医学院学报》2009年第3期24-26,共3页Journal of Henan University of Chinese Medicine

基　　金：国家自然科学基金(编号:3987099);广东医学院面上项目(编号:M2006061)

摘　　要：目的:建立半边旗中11β-羟基-15-氧-对映贝壳杉-16-烯-19-酸19-β-D-葡萄糖的含量测定方法。方法:采用HPLC法,ODSC18柱(25mm×4.6mm,5μm);流动相为甲醇-4mM的醋酸铵水溶液(2∶3),流速为1.0ml.min-1,检测波长为242nm。结果:11β-羟基-15-氧-对映贝壳杉-16-烯-19-酸19-β-D-葡萄糖在0.8～4.8μg范围内呈良好的线性关系(r=0.9998);半边旗中11β-羟基-15-氧-对映贝壳杉-16-烯-19-酸19-β-D-葡萄糖的含量,广东湛江产最高,为4.609mg·g-1生药,广东廉江最低为1.515mg·g-1生药。结论:本方法简便、可靠,适用于半边旗中11β-羟基-15-氧-对映贝壳杉-16-烯-19-酸19-β-D-葡萄糖的含量测定。Objective ： To establish an HPLC method for determination of 11β - hydroxy - 15 - oxo - ent - kaur - 16 - en - 19 - oic acid 19- β- D- glucoside in Pteris semipinnata L.. Methods： HPLC method was established. ODS C18 column was used, methanol-4 mM CH3COONH4 solution（2： 3）as mobile phase,at the flow rate of 1.0 ml · min^-1 and the UV detection wavelength at 242 nm. Resuits ：The linear of 11 β - hydroxy - 15 - oxo - ent - kaur - 16 - en - 19 - oic acid 19 - β - D - glucoside was 0.8 - 4.8 μg （ r = 0. 9998）. And the content of its was the highest in Pteris semipinnata L. from Zhangjiang, Guangdong（4. 609 mg · g^-1 ）, the lowest in Pteris semipinnata L. from Lianjiang, Guangdong. Conclusion： HPLC method is simple and reliable for determination of 11β - hydroxy - 15 - oxo - ent - kaur - 16 - en - 19 - oic acid 19 - β - D - glucoside in Pteris semipinnata L..

关 键 词：半边旗 11β-羟基-15-氧-对映贝壳杉-16-烯-19-酸19-β-D-葡萄糖 产地 HPLC 

分 类 号：R284.1[医药卫生—中药学]