出生前后全氟辛烷磺酸(PFOS)暴露对大鼠额叶皮质中IGF-Ⅰ、IGF-ⅠR和IGFBP-2 mRNA水平的影响  

Effects of Prenatal and Postnatal Perfluorooctane Sulfonate Exposure on IGF-Ⅰ、IGF-ⅠR and IGFBP-2 mRNA Levels in Frontal Cortex of Rat Pups

作　　者：刘利[1] 金一和[1,2] 王烈[3] 于红瑶[1] 刘薇[2] 于棋麟[1] 王柯[1] 刘冰[1] 王静[1] 

机构地区：[1]中国医科大学公共卫生学院卫生毒理学教研室,沈阳110001 [2]大连理工大学环境与生命学院工业生态与环境工程教育部重点实验室,大连116024 [3]中国医科大学公共卫生学院社会医学教研室,沈阳110001

出　　处：《生态毒理学报》2009年第2期190-196,共7页Asian Journal of Ecotoxicology

基　　金：国家自然科学基金项目(No.30771772);辽宁省教育厅计划项目(No.2008S058)

摘　　要：为研究出生前后不同时期全氟辛烷磺酸(PFOS)暴露对大鼠仔鼠额叶皮质胰岛素样生长因子-Ⅰ(IGF-Ⅰ)、胰岛素样生长因子-Ⅰ受体(IGF-ⅠR)和胰岛素样生长因子结合蛋白-2(IGFBP-2)mRNA水平的影响,将28只受孕Wistar大鼠按3:2:2的比例随机分到对照(C)、低剂量(L)和高剂量(H)组中,从妊娠第0天开始分别自由摄食含0、7.2、14.4mg·kg-(1以饲料中的PFOS计)的粉末状饲料进行连续染毒至出生后28天.采用交叉哺育的方法建立出生前后均不暴露(CC)、仅出生前暴露(LC和HC)、仅出生后暴露(CL和CH)、出生前后均暴露(LL和HH)于PFOS的动物模型.采用半定量RT-PCR法检测各组仔鼠额叶皮质IGF-Ⅰ、IGF-ⅠR和IGFBP-2mRNA水平的变化.结果表明,对照组仔鼠额叶皮质IGF-Ⅰ、IGFBP-2mRNA在PD14时处于较高水平,而在PD1和PD28时水平较低;IGF-ⅠRmRNA在PD1时只处于较低水平,随年龄的增加而逐渐升高.在PD1时,暴露组仔鼠IGF-Ⅰ、IGF-ⅠR、IGFBP-2mRNA水平显著高于对照组(p<0.05).在PD14时,除LC组仔鼠IGF-ⅠRmRNA水平显著高于对照组之外,其余各暴露组仔鼠3者mRNA水平均显著低于对照组(p<0.01).此外,出生后暴露PFOS组的仔鼠额叶皮质IGF-Ⅰ、IGFBP-2mRNA水平降低程度比出生前暴露PFOS组仔鼠更高.在PD28时,暴露组仔鼠IGF-Ⅰ、IGF-ⅠRmRNA水平显著低于对照组(p<0.01),而IGFBP-2mRNA水平与对照组之间的差异无显著性(p>0.05).出生前后不同时期暴露PFOS导致大鼠仔鼠额叶皮质IGF-Ⅰ、IGF-ⅠR和IGFBP-2mRNA水平变化,为PFOS发育神经毒性的研究提供了一定的线索.To study the effects of prenatal and postnatal perfluorooctane sulfonate （PFOS）exposure on insulin-like growth factor-Ⅰ （IGF- Ⅰ ）, insulin-like growth factor- Ⅰ receptor （IGF- Ⅰ R） and insulin-like growth factor binding proteins-2 （IGFBP-2） .mRNA levels in frontal cortex of rat pups. twenty-eight pregnant Wistar rats were randomly divided into control group （C）, low dose group （L）and high dose group （H）three groups in proportion of 3：2：2, which respectively received 0, 7.2, 14.4mg ·kg^-1 （calculated with PFOS in feed） from pregnancy day 0 to PD28 by free feeding. The animal models of prenatal and postnatal non-exposure （CC）, prenatal exposure （LC and HC）, postnatal exposure （CL and CH）, and prenatal and postnatal exposure （LL and HH）to PFOS were established using cross-fostering method. The IGF- Ⅰ , IGF- Ⅰ R and IGFBP-2 mRNA levels in frontal cortex of rat pups were measured using semi-quantitative RT-PCR. Results showed that the IGF- Ⅰ and IGFBP-2 mRNA levels in frontal cortex of control pups were higher at PD14 compared with the levels at PD1 and PD28; the IGF- Ⅰ R mRNA was at lower level at PD1, and increased with age. At PD1, IGF- Ⅰ , IGF- Ⅰ R and IGFBP-2 mRNA levels of exposure groups increased in comparison with that in control group significantly（p〈0.05 ）. At PD14, IGF- Ⅰ, IGF- Ⅰ R and IGFBP-2 mRNA levels of the other exposure groups were significantly lower than control （p〈0.01） excepted for the IGF- Ⅰ R mRNA level of LC group which was higher than control significantly. In addition, the decreased degree of IGF- Ⅰ and IGFBP-2 mRNA levels in postnatal exposure groups were more serious than that in prenatal exposure subjects： At PD28, IGF- Ⅰ and IGF- Ⅰ R mRNA levels of exposure groups Were significantly lower than control （p〈0.01）. However, there was no significant difference in IGFBP-2 mRNA level between exposure and control groups （p〉0.05）. Prenatal and postnatal exposure to PFOS resulted in the

关 键 词：全氟辛烷磺酸 额叶皮质 胰岛素样生长因子-Ⅰ 胰岛素样生长因子-Ⅰ受体 胰岛素样生长因子结合蛋白-2 

分 类 号：R114[医药卫生—卫生毒理学] X171.5[医药卫生—公共卫生与预防医学]