角膜缝线与碱烧伤诱导兔角膜新生血管的实验观察  

Corneal neovascularization induced by suture and alkali burn in rabbits:an experimental study

作　　者：韩旭光[1,2] 吴欣怡[1] 曲飞[3] 孙嬿[2] 王旭[2] 徐湘辉[2] 

机构地区：[1]山东大学齐鲁医院眼科,济南250012 [2]济南市第二人民医院眼科,济南250001 [3]山东大学附属济南中心医院,济南250014

出　　处：《山东大学耳鼻喉眼学报》2009年第2期79-82,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

摘　　要：目的利用角膜缝线、碱烧伤两种常用方法来诱导兔角膜新生血管(CNV)产生。观察CNV产生早期的过程中,其生长情况及血管内皮生长因子(VEGF)在兔角膜中的表达情况。方法将新西兰白兔75只随机分为3组,A组-正常对照组5只;B组-角膜缝线组35只;C组-碱烧伤组35只。每日裂隙灯显微镜观察角膜炎性反应情况与混浊程度,检测第3天、7天、14天角膜新生血管长度和数量并摄影记录;B、C两组分别于实验后第1、2、3、5、7、10、14天观察、取材。免疫组织化学(IHC)检测VEGF在兔角膜中的表达情况。结果伤后3 d,新生血管开始侵入角膜;7 d,新生血管生长活跃;10 d,新生血管达到生长高峰;14 d后B组与C组新生血管均出现回退迹象。角膜缝线组CNV面积明显小于碱烧伤组(P<0.01),10 d后VEGF在碱烧伤组的表达明显高于缝线组(P<0.01)。结论碱烧伤组相对于缝线组可以诱导产生更多的新生血管(CNV面积),且CNV退化的时间晚,这些可能与VEGF的表达有关;但角膜缝线更容易控制CNV产生的范围。Objective To explore the growth of corneal neovascularization induced by suture and alkali bum and to explore expression of vascular endothelial growth factor （VEGF）. Methods All animals（ n = 75） were randomly divided into three groups： the control group （5 rabbits）, the suture group（35 rabbits） and the alkali bum group （35 rabbits）. Biomieroscopie features ineluding length and area of corneal neovascularization were observed. Immtmoreaction of VEGF was detected on the 1st, 2nd, 3rd, 5th, 7th, 10th an 14th day after the operations. Results Corneal neovaseularization began to invade the cornea on day 3 and flourished on day 7, then reached its developmental peak on clay 10, and gradually regressed after day 14. The CNV area of the suture group was significantly smaller than that of the alkali bum group. In CNV tissues, positive immune reaction of VEGF was most apparent during the 3rd to the 7th day, and then remarkably declined on the 10th day. But positive immune reaction of VEGF of the suture-induced group declined more quickly than that of the alkali burn group. Conclusion In this study, alkali burn induced more CNV, which slowly declined, and suture had better controllability for area of CNV.

关 键 词：角膜新生血管 碱烧伤 角膜缝线 血管内皮生长因子 

分 类 号：R722.2[医药卫生—儿科]