HPLC法测定妇康宝口服液中芍药苷的含量  

Determination of peoniflorin in Fukangbao oral solution by HPLC

作　　者：齐伟[1] 邬丽红[1] 郑东明[1] 

机构地区：[1]武警医学院附属医院药剂科,天津300162

出　　处：《武警医学院学报》2009年第5期437-438,共2页Acta Academiae Medicinae CPAPF

摘　　要：【目的】建立HPLC法测定妇康宝口服液中芍药苷的含量。【方法】采用C18色谱柱（250mm×4．6mm，5μm），甲醇-0．05％磷酸二氢钾溶液（26：74）为流动相，检测波长243nm，流速1．0ml／min，柱温为30℃。【结果】芍药苷在0．04～0．4μg范围内具有良好的线性关系（r=0．9998），平均回收率为99．38％，RSD为1．96％（n=6）。【结论】该方法简便、快速、准确，可作为妇康宝口服液的质量控制方法。[ Objective] To establish the quality standard of Fukangbao oral solution. [Methods] HPLC analysis was carried out on C18 column （250 mm ×4.6mm,5μm） with methanol- 0.05% potassium dihydrogen phosphate solution （26： 74）, The flow rate was 1.0 ml/min, the detective wavelength was 243 nm. [Results] The linear range of peoniflorin was within 0.05 - 0.5 μg. The average recovery was 99.38% with RSD1.96% （ n = 6） . [ Conclusions] The method established in this paper is simple, rapid and accurate, hence, it could be used for the quality control of Fukangbao oral solution.

关 键 词：妇康宝口服液 芍药苷 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]