野生二粒小麦籽粒含硒量的差异分析  

Variance Analysis of Grain Selenium Contents in Wild Emmer Wheat,Triticum dicoccoide

作　　者：张翠炫[1] 严俊[1,2] 程剑平[1] 秦海波[3] 陈国雄[4] T.FAHIMA 

机构地区：[1]贵州大学麦作研究中心,贵阳550025 [2]海法大学进化研究所,以色列海法31905 [3]中国科学院地球化学研究所环境地球化学国家重点实验室,贵阳550002 [4]中国科学院寒区旱区环境与工程研究所,兰州730000

出　　处：《热带亚热带植物学报》2009年第3期229-236,共8页Journal of Tropical and Subtropical Botany

基　　金：贵州省国际合作重点项目(黔科合外G字(20064)001020);贵州省“十一五”重点攻关项目(黔科合NY(2006)3002F);贵州省第六批优秀青年科技人才培养计划资助

摘　　要：采用氢化物．原子荧光法，对种植于黔中的来自以色列15个野生二粒小麦群体110个基因型籽粒含硒量进行检测分析。结果表明，15个群体含硒量差异极显著（P≤0．001）；110个基因型籽粒硒含量和单粒硒含量分别为0．043～0．409mgkg^-1和0．008～0．125mg seed^-1，平均值分别为0．180mgkg。和0．046mg seed^-1籽粒硒含量和单粒硒含量最低的基因型分别是Gamla群体的TZ120和Mt．Hermon群体的TZ8，最高的基因型分别为Bat—Shelomo群体的TZ36和TZ34。在群体水平上硒浓度差异明显，变异系数CV为9％～74％；斯皮尔曼秩相关分析表明，籽粒硒含量分别与该群体起源地海拔、年平均降雨量、平均干旱天数呈显著负相关，与年均温、8月均温、1月均温呈显著正相关。单粒硒含量的相关分析与籽粒硒含量的相似。野生二粒小麦籽粒含硒量的差异是长期适应环境的结果，其遗传多样性将为小麦硒营养机理研究和育种利用提供材料。The grain selenium （Se） contents of 110 genotypes of the tetraploid wild progenitor of wheat （ Triticum dicoccoides） originating from 15 populations in Israel were detected by hydride generation atomic fluorescence spectrometry （HG-AFS）. The results showed that there were significant differences of grain Se content among populations by one way ANOVA （P≤0.001）. Grain Se concentration （GSeC） and grain Se content among the 110 wild emmer wheat genotypes varied from 0.043 to 0.409 mg kg^-l and 0.008 to 0.125 mg seed^-1, with the average of 0.180 mg kg^-1 and 0.046 mg per seed, respectively. The highest genotypes of GSeC and grain Se content were TZ36 and TZ34, both derived from Bat-Shelomo population, while the lowest genotypes were TZ120 from Gamla and TZ8 from Mt. Hermon, respectively. The coefficient of variation of GSeC among populations also was obviously different, ranged from 9% （Bat-Shelomo） to 74% （Givat-Koach）. According to Spearman＇s Rho Correlation analysis, the GSeC had significant negative correlationships with altitude, mean annual rainfall, mean number of dry days in original area, and positive correlationships with mean annual temperature, mean temperature in August, mean temperature in January and soil types, respectively. The grain Se content had similar tendency of GSeC. These demonstrated that natural selection has created abundant phenotypes of grain Se content in this wild species, and would be used for identification of novel genotypes or genetic studies on wheat mineral nutrition.

关 键 词：以色列 野生二粒小麦 籽粒硒含量 单粒硒含量 生态地理因素 

分 类 号：S512.1[农业科学—作物学]