大剂量肾上腺素对血管内皮细胞表达血管性血友病因子的影响  

THE INFLUENCE OF HIGH DOSE OF ADRENALINE ON vWF EXPRESSION IN VASCULAR ENDOTHELIAL CELL

作　　者：冼健坤[1] 陈蒙华[1] 谢露[2] 阮红[1] 黎静[2] 

机构地区：[1]广西医科大学第一附属医院心血管病研究所,南宁530021 [2]广西医科大学生理学教研室

出　　处：《广西医科大学学报》2009年第2期169-171,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助课题(30660229);广西自然科学基金资助课题(桂科自0728125和0229033);广西医疗卫生资助项目(桂卫重200632;Z2006057);广西大型仪器协作共用网资助

摘　　要：目的:探讨大剂量肾上腺素(Adrenaline,Adr)对血管内皮细胞(VEC)表达血管性血友病因子(vWF)的影响。方法:注射Adr造成大鼠VEC损伤,ELISA法测定大鼠血浆vWF水平;免疫组化法检测大鼠肺组织vWF的表达;体外培养人脐静脉内皮细胞(HUVEC),ELISA法测定HUVEC培养液vWF含量。结果:造模第4天和第5天,模型组大鼠血浆vWF水平均高于对照组(P<0.05),模型组肺组织免疫组化灰度值低于对照组(P<0.05),显示模型组肺组织vWF表达高于对照组。在HUVEC培养实验中,24、48h模型组培养液vWF水平均高于对照组(P<0.05)。结论:大剂量Adr可致大鼠VEC损伤标志物vWF表达增多。Objective： To study the influence of high dose of adrenaline （Adr） on vWF expression in vascular endothelial cell （VEC）. Methods.VEC was injured by injecting adrenaline in rats, the level of vWF in rat plasma was measured by ELISA and the vWF exprission in lung tissue was detected by immunohistochemistry. HUVEC was cultured in vitro, the content of vWF in supernatant was measured by ELISA. Results：The level of plasma vWF in model group was higher than that in control group （ P〈 0.05）. The grey-value of lung tissue in model group was lower than that in control group with immunohistochemistry analysis in the 4^th and 5^th days. In the cultured HUVEC, the supernatant vWF level in model group was higher than that in control group （ P 〈0.05） on the 24 and 48 h. Conclusion： High dose of Adr increases the expression of vWF, a VEC injury marker.

关 键 词：血管内皮细胞(VEC) 血管性血友病因子(vWF) 肾上腺素(Adr) 

分 类 号：R554.1[医药卫生—血液循环系统疾病]