Rf-1位点CAPS标记对水稻不同胞质雄性不育育性恢复系的关系分析  

Relationship among Fertility Restorers of Various CMS Systems Based on CAPS Markers Located at Rf-1 in Rice

作　　者：谭亚玲[1] 王石华[1] 洪汝科[1] 文建成[1] 郭艾[1] 寇淑燕[1] 金寿林[1] 谭学林[1] 

机构地区：[1]云南农业大学稻作研究所,昆明650201

出　　处：《分子植物育种》2009年第3期461-464,共4页Molecular Plant Breeding

基　　金：教育部博士点基金项目(20070676001);云南省科技厅十一五科技攻关项目(2006NG05)资助

摘　　要：水稻育性恢复基因Rf-1是目前水稻上克隆的唯一恢复基因。通过位于Rf-1位点的两个特异性CAPS(cleavable amplified polymorphic sequences)标记对滇Ⅰ型、野败型、红莲型及BT型恢复系基因型分析,发现这四种不同胞质的恢复系在Rf-1位点具有相同的带型,滇Ⅰ型的两个保持系具有不同的带型。结果表明这四种不同胞质系统的恢复系均具有Rf-1基因。该结论与从恢复系选育的系谱分析的结果相一致的。Fertility restorers used in different hybrid rice based on cytoplasmic male sterility （CMS） ofDian-type- 1 （DI）, Wild abortive （WA）, Hong-lian type （HL）, and Boro-type （BT）, were genotyped with two cleavable amplified polymorphic sequences （CAPS） markers located within Rf-1 gene, which is the only cloned fertility-restoring-gene in rice. The results showed that the restorers had the same DNA band pattern, but maintainer of CMS lines for CMS-D1 system had different band pattern. The results revealed that the restorers share the same fertility restoring （Rf） gene, R f-1. The results were consisted with pedigree analysis of Rf genes.

关 键 词：水稻 育性恢复基因 细胞质雄性不育系 Rf-1位点 CAPS标记 

分 类 号：S511[农业科学—作物学]