矮孟牛及其后代育成品种的多态性分析与指纹鉴定  

Genetic Diversity Analysis and Fingerprint Identification of ‘Aimengniu’ and its Deritives

作　　者：肖静[1] 王珊珊[1] 李秀全[2] 刘金良 田纪春[1] 

机构地区：[1]山东农业大学作物生物学国家重点实验室小麦品质育种室,泰安271018 [2]中国农业科学院作物科学研究所作物种质资源保护与研究中心,北京100081 [3]汶阳镇农业技术推广站,肥城271606

出　　处：《分子植物育种》2009年第3期483-489,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30671270);科技部863专项(2006AA1021E9)共同资助

摘　　要：为深入了解矮孟牛及其衍生品种(系)的遗传特性,为育种工作中合理利用小麦育种骨干亲本提供理论依据,本研究利用22对SSR引物分析了矮孟牛及其衍生品种(系)共25份材料的遗传多样性。在25份材料中共检测到128个等位变异,等位变异数目为3～9个,每个位点上平均等位变异数为5.82个。25份材料间的遗传相似性系数(GS)在0.43～0.90,平均为0.63,表明品种间遗传差异较小。用非加权配对算术平均法UPGMA将25份材料分为五类,聚类结果较好地反映了品种(系)间的亲缘关系。利用其中3对多态性较好的引物可以对供试材料的DNA指纹进行鉴定,为我国小麦品种的指纹图谱构建及品种权保护提供参考。Genetic diversity analysis of Aimengniu and its deritives was investigated with 22 pairs of SSR primers to detect the inherited characteristics and exploit these materials fully. A total of 128 alleles were detected among 25 materials, and the number of alleles for each primer pair ranged from 3 to 9 with an average of 5.82. Genetic similarity among the 25 materials varied from 0.43 to 0.90 with an average of 0.63, indicating that the genetic diversity was not abundant. 25 wheat cultivars （lines） fell into four clusters by UPGMA analysis, which could reflect the relationship among them. DNA fingerprints of experiment materials were constructed with 3 pairs of SSR primers. Which provided a reference for the building of fingerprint spectrum and protection of variety rights.

关 键 词：SSR 遗传多样性 指纹鉴定 矮孟牛 

分 类 号：S512.1[农业科学—作物学]