大白菜紫色性状的SRAP连锁标记的筛选  

SRAP Markers Linked to Purple Trait in Chinese Cabbage

作　　者：郁有健[1,2] 张耀伟[1] 张德双 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]北京蔬菜研究中心,北京100097

出　　处：《分子植物育种》2009年第3期573-578,共6页Molecular Plant Breeding

基　　金：北京市自然基金项目(6092009);北京市农林科学院青年基金共同资助

摘　　要：以紫色大白菜BC1回交群体的120个单株为材料,采用混合集群分析法构建大白菜的紫色与非紫色池,应用SRAP分子标记技术筛选两池间的多态性,并对与紫色性状相关的QTL和对应的连锁关系进行了初步分析。结果表明,在144对SRAP引物组合中,仅有8对引物组合可以在两池间扩增出较稳定的多态性产物。应用获得的8对引物组合对120个单株的DNA进行SRAP分析,初步获得3个与紫色性状相关的QTL:qp-c1-1、qp-c1-2和qp-c1-3,同时获得6个与这3个QTL连锁的标记,其中m7e11-490、m6e8-210、m7e11-900n和m6e8-100n4个标记与其最近QTL位点的连锁距离分别为0.1cM、0.1cM、0.2cM和2.2cM。这为紫色白菜的分子辅助育种提供了科学依据。In order to study QTL traits related to purple genes in Chinese cabbage （Brassica campestris L. ssp. pekinensis）, 120 BC1 progenies were used to construct purple and non-purple gene pools by using bulked segregation analysis （BSA）. The polymorphism between the two gene pools was selected by SRAP. Also, QTL related to purple trait and their linkage relationship were analyzed prelimarily. Results showed that only 8 of the 144 primer pair combinations could amplify polymorphic bands stably between the two gene pools. 3 QTLs （qp-c 1-1, qp-c 1-2 and qp-cl-3） related to purple trait were obtained in 120 individuals by SRAP with the 8 primer pairs. Out of 6 markers linked with the 3 QTLs, 4 markers （m7el 1-490, m6e8-210, m7el 1-900n and m6e8-100n） were found to be linked with their nearest QTLs at a distance of 0.1 cM, 0.1 cM, 0.2 cM and 2.2 cM, respectively. This provides scientific basis for marker-assisted breeding of purple Chinese cabbage.

关 键 词：大白菜 紫色性状 SRAP QTL 

分 类 号：S634.1[农业科学—蔬菜学]