落叶松多倍体体细胞胚的高效诱导途径  

Polypliod Induction of Larix by Somatic Embryogenesis

作　　者：张清国[1,2] 齐力旺[2] 韩素英[2] 梁国鲁[1] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]中国林业科学研究院林业研究所,北京100091

出　　处：《分子植物育种》2009年第3期629-634,共6页Molecular Plant Breeding

基　　金：国家863项目(2006AA100109;2008AA10Z126);国家自然科学基金重点项目(30830086);国家973项目(2009CB119100);国家林业局948创新项目(2006-4-C01)共同资助

摘　　要：以日本落叶松×华北落叶松杂种无性系胚性细胞系Y68为材料,通过含有不同浓度的秋水仙素和氨磺灵水溶液、固体培养基和液体培养基进行处理,以体细胞胚分化能力和多倍体体细胞胚获得率为参数进行了对比研究。结果表明:秋水仙素水溶液浸泡法处理对落叶松多倍体体细胞胚诱导效果最显著;先对细胞系处理再进行体细胞胚分化较适合落叶松多倍体体细胞胚的发生。在500mg/L的秋水仙素水溶液中浸泡36h情况下多倍体诱导率最高,达79.8%,体细胞胚分化率为217.1个/g。Using embryogenic cell line Y68 ofLarix kaempferix×L, principis as material, comparisons on frequency of somatic embryogenesis and the induction ratios of polyploid were carried out by various treatment including different concentration of colchicine and oryzalin, solid medium and liquid medium. The results showed that immersing somatic embryogenic callus in colchicines solution before somatic embryos induction was the most effective way for polypliod induction ofLarix. Pretreatment of the cell lines was more suitable for somatic embryogenesis of Larix. The induction ratios reached as high as 79.8% with number of somatic embryogenesis was 217.1 per gram of inoculated cell by 500 mg/L colchicine solution treatment for 36 h.

关 键 词：落叶松 体细胞胚发生 多倍体 秋水仙素 氨磺灵 

分 类 号：S791.22[农业科学—林木遗传育种] S565.203.5[农业科学—林学]