转基因银腺杂种杨对土壤微生物的影响  

Effects of Transgenic Populus alba×P.glandulosa on Soil Microorganism

作　　者：侯英杰[1] 苏晓华[1] 焦如珍[1] 黄秦军[1] 褚延广[1] 

机构地区：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091

出　　处：《林业科学》2009年第5期148-152,共5页Scientia Silvae Sinicae

基　　金：国家"十一五"科技支撑计划课题(2006BAD01A15);国家"948"引进国际先进林业科学技术创新项目(2006-4-C01)

摘　　要：In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.In this study, genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba x P. glandulosa and the soil samples from the stands were collected in two successive years, and the bacteria, fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile, significant differences of the soil microorganism community were found among several transgenic lines, and between a few transgenic and non-transgenic lines, but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.

关 键 词：转基因银腺杂种杨 土壤微生物 生态安全 

分 类 号：S714.3[农业科学—林学]