鹅Pit-1基因部分序列多态性分析  

Analysis on the Polymorphism of Sequences of Pit-1 Gene in Goose

作　　者：程金花[1] 赵文明[1] 乔娜[1] 王学斌[1] 姜庆林 张康宁 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]广东省家禽研究所,广州510520 [3]常州市立华畜禽有限公司,常州213168

出　　处：《畜牧兽医学报》2009年第5期658-663,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技部支撑计划重点项目(2006BDA14B06);国家科技部支撑计划重大专项(2006BDA01A09);江苏省属高校自然科学基础研究面上项目(07KJB230138);国家水禽产业技术体系建设项目

摘　　要：根据鸡Pit-1 mRNA和基因组序列设计1对引物,以籽鹅、狮头鹅、皖西白鹅、四季鹅、浙东白鹅和朗德鹅6个群体的DNA为模板,扩增鹅Pit-1基因的部分片段。结果发现该片段存在2个插入/缺失突变,共出现3种基因型,分别为AA、AB和BB;与AA基因型相比,BB基因型分别在扩增片段的132和145 bp后插入3和13 bp。所有群体在该突变位点上的等位基因频率均处于哈代-温伯格平衡状态(P>0.05);朗德鹅群体基因型分布与其他5个鹅群体均存在极显著差异(P<0.01);籽鹅和狮头鹅群体内基因型的分布与其他3个鹅群体存在显著差异(P<0.05)。在狮头鹅和朗德鹅群体内,等位基因A为优势等位基因,而在皖西白鹅、四季鹅和浙东白鹅群体内等位基因B为优势等位基因。Genetic polymorphism of goose Pit-1 gene fragment was analyzed in 6 goose populations. The primer was designed according to chicken Pit-1 mRNA and genomic sequence. Two insertion/deletion mutations were found in the sequence of Pit-1 gene, and 3 genotypes AA, AB and BB were detected in 6 goose populations. Compared with AA genotype, there were 3 and 13 bp insertions in BB genotype after position 132 and 145 bp,respectively. All the populations were in Hardy-Weinberg equilibrium at this polymorphic site （P〉0.05）. The genotype distribution in Landosie was significantly different with that in other goose populations （P〈0.01）. The genotype distribution in Zi goose and Shitou goose were significantly different with that in other populations（P〈0.05）. Allele A was the dominant allele in Shitou and Landoise goose populations, while allele B was the dominant allele in Wanxi White goose, Siji goose and Zhedong White goose populations.

关 键 词：鹅 垂体特异性转录因子 多态性 

分 类 号：S835[农业科学—畜牧学] Q343.13[农业科学—畜牧兽医]