采用AFLP和ERIC-PCR技术研究规模化鸡场健康鸡群中产气荚膜梭菌的遗传多样性  

Genetic Diversity of Clostridium perfringens Isolated from Healthy Chickens at Commercial Farms Revealed by AFLP and ERIC-PCR

作　　者：倪学勤[1,2] 郑晓丽[1,4] 曾东[1,2] Joshua Gong 宋振银[1,4] 

机构地区：[1]四川农业大学动物医学院动物微生态研究中心,雅安625014 [2]动物疫病与人类健康四川省重点实验室,雅安625014 [3]Food Research Program,Agriculture and Agri-Food Canada,Guelph,Ontario,Canada N1G 5C9 [4]四川农业大学资源环境学院,雅安625014

出　　处：《畜牧兽医学报》2009年第5期717-724,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川省科技厅国际合作项目(HH20080016)

摘　　要：为了调查产气荚膜梭菌在健康鸡群中的流行现状,掌握我国部分地区规模化鸡场产气荚膜梭菌的遗传多样性,分析其流行特点与地区差异的关系,利用AFLP(amplified fragment length polymorphism)和ERIC-PCR(en-terobacterial repetitive intergenic consensus PCR)方法对从四川省8个市10个规模化鸡场分离得到的34株A型产气荚膜梭菌进行基因型分析。结果表明,用AFLP技术将34株菌分为12个亚型,用ERIC-PCR方法分为8个亚型。分析亚型分布情况发现:不同鸡场产气荚膜梭菌的亚型差异明显;而同一鸡场的亚型较简单,以一种亚型为主,交叉有少数其他亚型;优势基因型分别为AFLP基因Ⅷ型或ERIC-PCR基因1型。该研究结果说明:四川省规模化鸡场健康鸡群中产气荚膜梭菌的遗传多样性较低,其流行特点与地区差异相关。The objective of this research was to study the epidemiology of C. perfringens colonizing healthy birds, characterize the population diversity and observe how diversity changed between different areas. Isolates were obtained from fresh feces and cecal samples of healthy chicken in ten farms of 8 cities in Sichuan province. Each isolate was typed using amplified fragment length polymorphism （AFLP） and enterobacterial repetitive intergenic consensus PCR （ERIC-PCR）. A total of 34 C. perfringens isolates of type A from 600 birds were typed. There found 12 AFLP genotypes and 8 ERIC-PCR genotypes. The analysis of genetic type and origin source revealed that isolates from different farms had different genetic types. The subtype of C. perfringen appeared to be simple within a single farm, and mixed with few other gene subtypes. Thedominant gene type of C. perfringen was AFLP gene type Ⅷ or ERIC-PCR gene type Ⅰ. These results reveal that the diversity of C. perfringen of healthy chickens in Sichuan province is low and there is close correlation between the epidemiology and regions.

关 键 词：产气荚膜梭菌 健康鸡群 遗传多样性 AFLP ERIC-PCR 

分 类 号：S852.616.3[农业科学—基础兽医学]