内蒙古地方品种绵羊朊蛋白基因多态性分析  

Analysis on Polymorphisms of the Prion Protein Gene in Local Sheep Species of Inner Mongolia,China

作　　者：王伊琴[1,2] 秦贞奎[3] 包勇敢 寇福军 荆文奎 乔俊文[1] 赵德明[1] 

机构地区：[1]中国农业大学动物医学院国家动物海绵状脑病实验室,北京100193 [2]内蒙古二连浩特出入境检验检疫局,二连浩特011100 [3]中国检验检疫科学研究院,北京100123

出　　处：《畜牧兽医学报》2009年第5期780-784,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技部973专项(2005CB523000);国家科技支撑计划(2006BAD06A13)资助项目

摘　　要：对中国内蒙古的乌珠穆沁羊(46)、苏尼特羊(34)和蒙古羊(22)进行朊蛋白基因的克隆和多态性分析。结果表明,发现了8个朊蛋白基因多态性位点,其中M157I、Q220H、R223K为未见报道的朊蛋白基因多态性位点;痒病中度易感的朊蛋白基因ARQ/ARQ占74.5%,具有痒病抗性朊蛋白基因ARR/ARR占7.9%,未检出对痒病高度易感的朊蛋白基因VRQ/VRQ。从基因水平上推论,乌珠穆沁羊携带的羊痒病抗性的ARR/ARR基因频率较高(15.2%),占ARR/ARR基因型的87.5%,发生羊痒病风险较低。该研究结果在中国活羊的出口贸易和品种选育方面有重要的意义,为出入境动物检疫中羊痒病的风险分析和风险评估提供重要的基础理论资料。We investigated prion protein gene（PRNP） polymorphisms in three sheep breeds in Inner Mongolia, China. Blood samples were collected from 46 Ujumqin, 34 Sunite and 22 Mongolian sheep. The genetic DNA of PRNP of each sheep was extracted, amplified, and sequenced, and amino acid alignment was determined. Polymorphism was detected at 8 codons, among which M157I, Q220H and R223K have not been previously reported. The frequency of the amino acid residues ARQ/ARQ at codons 136, 154 and 171, which is associated with medium-high susceptibility to scrapie, was 74.5%, and the frequency of scrapie-resistant amino acid residues ARR/ ARR was 7.9 %. The highly susceptible amino acid residues VRQ/VRQ at these codons were not detected from the tested sheep. Of the 3 sheep breeds, Ujumqin sheep had the highest frequency （15.2%） of scrapie-resistant amino acid sequence, ARR/ARR at codons 136, 154 and 171, accounting for 87.5 % sheep that carry these polymorphisms. Our findings are of special importance for both live sheep export and sheep breeding.

关 键 词：朊蛋白基因 基因多态性 易感性 抗性 朊病 羊痒病 

分 类 号：S852.659.7[农业科学—基础兽医学]