细胞聚集体复合支撑体体外构建人鼻翼形态组织工程软骨的实验研究  

Construction of tissue engineering cartilage with the shape of human nasal alar in vitro by using bone marrow stromal stem cells macroaggregate and internal support

作　　者：张浚睿[1] 陈富林[2] 魏建华[1] 封兴华[1] 毛天球[1] 

机构地区：[1]西安第四军医大学口腔医院颌面外科,710032 [2]西安西北大学生命科学院

出　　处：《实用口腔医学杂志》2009年第3期311-314,共4页Journal of Practical Stomatology

基　　金：国家“973”课题基金项目(编号:G1999054308);军队临床高新技术重大项目-骨与软骨组织工程技术

摘　　要：目的:尝试以骨髓基质细胞(bone marrow stromal cells,BMSCs)为种子细胞,利用细胞聚集体复合聚乳乙醇酸(PLGA)内支撑体,在外支架塑形以及生物反应器环境下构建人鼻翼形态软骨。方法:全骨髓培养法收集培养兔BMSCs,转化生长因子-β1(TGF-β1)诱导,高密度连续培养构建出膜状细胞聚集体,制作人鼻翼形态PLGA内支撑体,将细胞聚集体缠绕在其表面,并用金属外支架对复合体进行塑形,进入生物反应器动态培养8周。取材从大体形态、组织学特点、蛋白多糖(glycosam inoglycans,GAG)含量以及抗压性能等方面评价新生软骨的质量。结果:体外构建的组织工程软骨保持了预先设计的外形,外观及组织学结构均类似于天然软骨,含有丰富的蛋白多糖。结论:细胞聚集体复合支撑体能形成特定形态的软骨组织,形成的组织结构均匀,细胞表型维持良好,并为软骨缺损的修复提供了新的方法。Objective： To explore a novel strategy to fabricate tissue engineered cartilage with the shape of human nasal alar by using bone marrow stromal cells （BMSCs） macroaggregate and evaluate the feasibility of creating 3-D cartilage equivalents with internal support and external scaffold. Methods： Rabbit BMSCs were expanded and in- duced by transforming growth factor-1 to improve chondrocyte phenotype. BMSCs macroaggregates were obtained by continuous culture and wrapped by PLGA support in the shape of human nasal alar. The constructs were transferred into the porous metallic cabinet and incubated in spinner flask for 8 weeks and cartilage formation was investigated by gross inspection and histological, biochemical, biomechanical examinations. Results ： After in vitro culture, cartilage like tissues with human nasal alar shape had been constructed successfully. Histological analysis showed engineered cartilage consisted of evenly spaced lacunae embedded in proteoglycans rich matrix. Conclusion： Based on the cell macroaggregate and internal support strategy, 3-D cartilage with special shape can be successfully fabricated which provided a highly effective cartilage graft substitute and can be useful in many situations of cartilage defect encountered in clinical practice.

关 键 词：组织工程 骨髓基质细胞 鼻翼 软骨 

分 类 号：R318[医药卫生—生物医学工程]