人非小细胞肺癌与外周血淋巴细胞表达ERCC1的相关性研究  

ERCC1 expression in peripheral blood lymphocytes and non-small cell lung cancer

作　　者：郝萍[1] 赵伟鹏[1] 刁鑫伟[1] 王卫东[1] 关华军[1] 胡义德[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥医院全军肿瘤研究所,重庆400037

出　　处：《第三军医大学学报》2009年第12期1201-1204,共4页Journal of Third Military Medical University

摘　　要：目的探讨切除修复交叉互补基因1(excision repair cross-complementation group 1,ERCC1)在人非小细胞肺癌(non-small cell lung cancer NSCLC)癌组织和外周血淋巴细胞(peripheral blood lymphocyte,PBL)中的表达及其相关性,以期建立临床实用简便的肿瘤学指标。方法采用间接二步法免疫荧光化学染色检测47例Ⅱ期、Ⅲ期NSCLC患者癌组织和PBL中ERCC1的表达及健康人PBL中ERCC1的表达。结果ERCC1在癌组织、NSCLC患者PBL和健康人PBL中的表达分别为53.19%(25/47)、48.94%(23/47)和20%(4/20),其中癌组织的ERCC1表达与NSCLC患者PBL的表达呈正相关,20例健康人PBL的ERCC1表达阳性率显著低于NSCLC患者。ERCC1的表达与临床病理类型无关。结论检测NSCLC患者PBL的ERCC1表达可间接反应癌组织的ERCC1表达状况。Objective To explore the correlation of the expression of excision repai r cross-complementation group 1 （ERCC1） with non-small cell lung cancer （NSCLC） tissues and peripheral blood lymphocytes （PBLs）. Methods ERCC1 expressions in specimens and PBLs from 47 patients with Stage Ⅱ and Ⅲ NSCLC and healthy PBLs were detected with double immunofluorescence staining. Results The positive expression rates of ERCC1 were 53. 19% , 48.94% , and 20% in carcinoma tissues, PBLs of NSCLC, and PBLs of healthy people, respectively. The expression rate of ERCC1 in carcinoma tissues was higher than that in PBLs of healthy people （P 〈 0.05 ） and positively correlated with that in PBLs of the patients. Conclusion The expression of ERCC1 in PBLs of NSCLC patients can indirectly represent ERCC1 expression in carcinoma tissues.

关 键 词：切除修复交叉互补基因1 非小细胞肺癌 外周血淋巴细胞 

分 类 号：R331.144[医药卫生—人体生理学] R730.43[医药卫生—基础医学]