高糖和甲基乙二醛对血管周细胞增殖及Ang-1表达的影响  

Effects of high glucose and methylglyoxal on proliferation and expression of angiopoietin-1 of vascular pericytes

作　　者：张小娟[1] 方炜[1] 黄丛洋[1] 林爱武[1] 戴慧莉[1] 倪兆慧[1] 钱家麒[1] 

机构地区：[1]上海交通大学医学院仁济医院肾脏科上海市腹膜透析研究中心,上海200001

出　　处：《上海交通大学学报（医学版）》2009年第5期496-499,共4页Journal of Shanghai Jiao tong University:Medical Science

基　　金：国家自然科学基金(30600290);上海市科委青年启明星项目(07QA14040)~~

摘　　要：目的研究高糖和葡萄糖降解产物甲基乙二醛(MGO)对血管周细胞增殖及血管生成素-1(Ang-1)表达的影响。方法选用体外培养的第3代人脑血管周细胞作为实验细胞,在细胞培养液中分别加入1.5%、2.5%和4.25%葡萄糖(设立相应浓度的甘露醇对照组)及400μmol/L和800μmol/L MGO进行分组干预,以不含血清DMEM培养的细胞作为正常对照组。于处理后12、24、48和72 h,应用MTT法检测细胞增殖(D490 nm)。于处理后6 h和12 h,Real-time PCR法检测细胞Ang-1 mRNA表达;ELISA法检测细胞培养上清中Ang-1蛋白表达。结果4.25%葡萄糖组和甘露醇对照组、800μmol/L MGO组各时间点D490 nm均明显低于正常对照组(P<0.05)。除1.5%葡萄糖和甘露醇对照组外,其余各干预组Ang-1 mRNA表达均显著低于正常对照组(P<0.05)。各干预组相应时间点Ang-1蛋白表达均低于正常对照组;干预后6 h均低于干预后12 h;葡萄糖各浓度组均低于甘露醇对照组;800μmol/L MGO组低于400μmol/L MGO组;4.25%葡萄糖组低于1.5%和2.5%葡萄糖组;以上各组间比较差异均有统计学意义(P<0.05)。结论高糖和MGO以浓度依赖方式抑制体外培养的血管周细胞增殖,并下调Ang-1表达。Objective To investigate the effects of high glucose and glucose degradation product methylglyoxal （MGO） on the proliferation and expression of angiopoietin （Aug） -1 of vascular pericytes. Methods Cultured pericytes of the third passage were incubated with media containing 1.5%, 2.5% and 4.25% glucose （ mannitol control groups of the same concentration were also established）, 400 μmol/L and 800μmol/L methylglyoxal （MGO）, respectively. Cells incubated with serum-free DMEM media were served as normal control group. Proliferation of pericytes （D490nm） was assessed by MTT assay after incubation for 12, 24, 48 and 72 h. After incubation for 6 h and 12 h, the expression of pericyte Ang-1 mRNA was detected by Real-time PCR, and the expression of Ang-1 protein in the supernatant was detected by ELISA. Results D490nm of 4.25% glucose group, 4.25% mannitol control group and 800 μmol/L MGO group was significantly lower than that of normal control group （P 〈 0.05）. Except for 1.5% glucose group and 1.5% mannitol control group, the expression of Aug-1 mRNA of the other experimental groups was significantly lower than that of normal control group （P 〈 0.05）. The expression of Ang-1 protein of all the experimental groups was significantly lower than that of normal control group （ P 〈 0.05）, and that at 6 h was significantly lower than that at 12 h （P 〈0.05）. Besides, the expression of Ang-1 protein was significantly lower in glucose groups than that in mannitol control groups at the same concentrations （P 〈 0.05）, that of 800 μmol/L MGO group was significantly lower than that of 400 μmol/L MGO group （ P 〈 0.05）, and that of 4.25% glucose group was significantly lower than that of 1.5% and 2.5 % glucose groups （ P 〈 0.05）. Conclusion High glucose and MGO inhibit proliferation and down-regulate expression of Ang-1 of cultured vascular pericytes in a dose-dependent manner.

关 键 词：高糖 甲基乙二醛 血管生成素-1 血管周细胞 

分 类 号：R459.9[医药卫生—治疗学]