应用γH_2AX检测甲醛致DNA损伤的研究  

Study on Application of γH_2AX to Detecting Formaldehyde Induced DNA Damage

作　　者：刁汇玲[1] 高金祥[2] 赵冬梅[1] 马中女[1] 巩永凤[3] 

机构地区：[1]滨州医学院,山东滨州256603 [2]滨州医学院附属医院,山东滨州256603 [3]浙江大学生命科学院,浙江杭州310000

出　　处：《工业卫生与职业病》2009年第3期129-132,共4页Industrial Health and Occupational Diseases

基　　金：滨州医学院科技计划(2006KJ27)

摘　　要：目的探讨磷酸化的H2AX(即γH2AX)能否灵敏、快速、简便的检测甲醛致DNA损伤。方法用甲醛处理中国仓鼠肺细胞株(V79)肺细胞,应用免疫荧光实验检测γH2AX焦点的形成,并通过单细胞凝胶实验验证DNA损伤程度。结果20μmol/L甲醛处理V79细胞10 min即可诱导γH2AX焦点形成增加,但2 h时才出现彗尾增长。1μmol/L甲醛处理细胞8 h时,γH2AX平均焦点数及焦点细胞率与对照组相比明显增加,差异有统计学意义(P<0.05),但此浓度在所有时间段都不能导致彗尾增长。结论γH2AX可在早期监测甲醛对DNA的损伤,并能检测到低浓度的甲醛致DNA损伤,因此,其敏感性优于单细胞凝胶实验。Objective To investigate whether γH2AX is a sensitive, rapid and simple technique in detecting formaldehyde induced DNA damage. Methods After V79 lung cells were treated with formaldehyde, γH2AX foci formation was detected by immune-fluorescent microscopy. Single cell gel electrophoresis was employed to detect DNA damage. Results Increase of the formation of γH2 AX loci could be induced by treating V79 lung cells in 20 μmol/L formaldehyde for 10 min, however, the increase of comet tail length would increase only 2 h later. When the cells were treated with 1μmol/L formaldehyde for 8 h, the mean value of foci per cell and the percentage of γH2 AX foci cells increased significantly compared with the control （P 〈 0.05）, however, this dosage could not increase comet tail length at any time duration. Conclusions γH2AX could detect formaldehyde induced DNA damage at early stage, moreover, DNA damage induced by low concentration formaldehyde could also be detected. It is more sensitive than single cell gel electrophoresis.

关 键 词：γH 2AX 甲醛 DNA损伤 

分 类 号：R115[医药卫生—公共卫生与预防医学]